FastQCFastQC Report
Wed 8 May 2024
EGAF00004692592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808763
Total Bases60 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT47630.5889240729360765No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39680.49062580756043483No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32640.4035792933158416No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG30870.3816940191378686No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30290.3745225733620356No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT28260.3494225131466202No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27650.34188013052031313No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25560.3160381966039495No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG24950.3084958139776424No Hit
CTCCTATCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCT24090.29786229093071764No Hit
GTAATGCATTCCACTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT23860.2950184417437494No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGCATTCCACTACTTGGATTGG23750.2936583399586776No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23330.28846522405203995No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22750.281293778276207No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA22320.2759770167527446No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21790.2694237990610352No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21710.26843463412643753No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20910.258542984780461No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT20750.25656465491126573No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC20690.2558227812103175No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGCATTTA20250.2503823740700304No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA20150.2491459179017833No Hit
AGATAGGAGTTTGTAGAGCTTGCATGCAGTAATAAATGCCAACATCCTCA18840.23294834209774679No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18500.22874439112570677No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG18390.227384289340635No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG18110.22392221206954324No Hit
CTACAAACTCCTATCTTCGGCCAAGGGACACGACTGGAGATTAAACGAAC18010.2226857559012962No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC18010.2226857559012962No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG17950.22194388220034794No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17900.22132565411622443No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC17650.21823451369560676No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC17620.21786357684513263No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA17520.21662712067688555No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG16940.2094556749010526No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT16060.1985748606204784No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA15850.1959783026671596No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAATGCCAACATCCTCAGCCTCC15760.19486549211573723No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCC15570.19251622539606783No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGATA15460.19115612361099604No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGT15250.18855956565767723No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTATCTTCGGCCAAGGGACAC15150.18732310948943015No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCT15120.18695217263895603No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC15090.1865812357884819No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTG14910.1843556146856372No Hit
GTCGTGTCCCTTGGCCGAAGATAGGAGTTTGTAGAGCTTGCATGCAGTAA14360.17755510576027833No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTG14330.1771841689098042No Hit
CAGTAATAAATGCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGT14190.17545313027425832No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG13890.17174376176951717No Hit
TAGTAATGCATTCCACTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGT13750.17001272313397126No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13680.16914720381619833No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG13470.1665506458628795No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA13410.16580877216193124No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGA13400.16568512654510653No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13130.16234669489083947No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA13070.16160482118989122No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA12890.1593792000870465No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12710.1571535789842018No Hit
CTCCTGCATAGTAATGCATTCCACTACTTGGATTGGTACCTGCAGAAGCC12630.15616441404960416No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATTTCC12590.15566983158230532No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12590.15566983158230532No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12400.1533205648626359No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12280.15183681746073943No Hit
GTAATAAATGCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA12240.1513422349934406No Hit
GCATAGTAATGCATTCCACTACTTGGATTGGTACCTGCAGAAGCCAGGGC12080.1493639051242453No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11870.14676734717092646No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA11790.14577818223632882No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC11770.14553089100267938No Hit
AGTCAGAGCCTCCTGCATAGTAATGCATTCCACTACTTGGATTGGTACCT11660.14417078921760762No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT11640.14392349798395823No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11570.14305797866618528No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11360.14046142071286644No Hit
GTGGAGGCTGAGGATGTTGGCATTTATTACTGCATGCAAGCTCTACAAAC11260.13922496454461938No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG11220.13873038207732055No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11180.13823579961002172No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11150.13786486275954762No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT10900.13477372233892992No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA10870.13440278548845583No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10760.13304268370338404No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10730.13267174685290994No Hit
GAGTGGAGGCTGAGGATGTTGGCATTTATTACTGCATGCAAGCTCTACAA10660.131806227535137No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGT10610.13118799945101345No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10610.13118799945101345No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT10530.1301988345164158No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC10500.1298278976659417No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAGTAGTGGAATGCATTACTATGC10280.12710769409579817No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10220.12636582039484992No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC10190.1259948835443758No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG10130.12525300984342755No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9940.12290374312375814No Hit
ATGCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC9600.11869979215171811No Hit
CTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT9600.11869979215171811No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATC9590.11857614653489341No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT9510.11758698160029576No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCC9430.1165978166656981No Hit
GGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTCCACTCTCC9190.11363032186190516No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8830.10917907965621572No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8690.10744804102066983No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8690.10744804102066983No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGG8640.10682981293654631No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8630.1067061673197216No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8610.10645887608607218No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG8510.10522241991782512No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8480.104851483067351No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8230.10176034264673334No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8190.10126576017943452No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)