FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448534
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGGACAGCAAGCAGTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC77430.5345404388160719No Hit
GTCCTATAGTGGTCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC48670.3359948748182645No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44990.3105898791467787No Hit
GTAGTACACAGCCGTGTCCTCGGCTCTGAGGCTGTTCATTTGCAGATACA42590.2940214037088532No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA36650.25301442699998755No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33870.23382260961772386No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31920.22036072332440934No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA28110.1940582685667026No Hit
GCCTGAGGATGAGGCTGAATATTACTGCTTGCTGTCCTATAGTGGTCCTTGGGTGTTCGGCGGAGGGACCAAG27190.18770701964883119No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA26990.18632631336233738No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAATCGGGCAGGGACC26900.18570499553341516No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26660.18404814798962263No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC26170.18066541758771282No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGACCACTATAGGACAGCAAGCAGTAATATTCAG26140.18045831164473874No Hit
CCACTGGAGCTGTCACCAGTGGTCATTATCCCTGCTGGTTCCAGCAGAAG23610.16299237712059225No Hit
GAATATTACTGCTTGCTGTCCTATAGTGGTCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG23330.16105938831950095No Hit
GTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG23170.15995482329030591No Hit
GTCCTATAGTGGTCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT22720.15684823414569488No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22510.15539849254487642No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21640.1493924201986284No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21530.14863303174105683No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21470.14821881985510868No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21410.14780460796916056No Hit
CTCCTGGGTCCCTGCCCGATTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC20310.14021072339344468No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20170.13924422899289904No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20070.13855387584965215No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19490.13454982761882012No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19430.134135615732872No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19410.1339975451042226No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19300.13323815664665103No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19240.1328239447607029No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18840.1300625321877153No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18670.1288889318441956No Hit
GTGTACTACTGTGCGACTTGGGGGGGGGATTGGTTCGCCCCCTGGGGCCA18560.128129543386624No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18410.12709401367175366No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18260.1260584839568833No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGACCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC18030.12447067172741545No Hit
CCCAAGGACCACTATAGGACAGCAAGCAGTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGG17610.12157118852577847No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17600.12150215321145379No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16720.1154270455508811No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15970.11024939697652936No Hit
GAAGAAACACTCCTGGGTCCCTGCCCGATTCTCAGGCTCCCTCCTTGGGG15720.10852351411841214No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15580.10755701971786648No Hit
CCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCC15520.10714280783191833No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15490.10693570188894427No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15390.10624534874569738No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15270.1054169249738011No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15230.10514078371650233No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14510.10017024108512468No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14500.10010120577079999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1500.059.74608641
TCTCGTA1300.055.4280138
TCGTATG1700.050.6079740
CTAGCGC2850.048.9233228
TAGCGCT3000.047.63215329
ACTAGCG2950.047.24040227
GGTATCA11850.047.003721
ATGCCGT1700.046.83957744
TCTGGAA16550.045.98996770
TACGTAT804.1772233E-544.4587670
GACTAGC3150.044.22267526
CGAGACT3150.044.1936723
ACGAGAC3250.043.87859322
CGCTCAT2650.043.76413732
GCGCTCA3450.041.47665431
ATCTCGT1850.040.74728437
CCCACGA3700.038.5406819
ATCTCCG3750.038.02548610
GTATCAA31000.037.5784721
GCCGTCT1950.037.4127346
Produced by FastQC (version 0.11.5)