FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448534
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGGACAGCAAGCAGTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC71730.4951903096509989No Hit
GTCCTATAGTGGTCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC50010.34524560693777295No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42330.2922264855364113No Hit
GTAGTACACAGCCGTGTCCTCGGCTCTGAGGCTGTTCATTTGCAGATACA40070.2766245044990315No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36170.2497007319124025No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33620.2320967267596066No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA32900.22712618412822896No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30940.2135952625205898No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA28960.19992627028430124No Hit
GCCTGAGGATGAGGCTGAATATTACTGCTTGCTGTCCTATAGTGGTCCTTGGGTGTTCGGCGGAGGGACCAAG27860.19233238570858538No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26380.1821151591885313No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25970.17928471130121904No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25430.17555680432768578No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAATCGGGCAGGGACC24640.1701030144960353No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC24320.16789388443764522No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGGACCACTATAGGACAGCAAGCAGTAATATTCAG24240.1673416019230477No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23750.16395887152113792No Hit
GAATATTACTGCTTGCTGTCCTATAGTGGTCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG23380.16140456489112442No Hit
GTCCTATAGTGGTCCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT23110.1595406114043578No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22380.15450103345865543No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22110.1526370799718888No Hit
CCACTGGAGCTGTCACCAGTGGTCATTATCCCTGCTGGTTCCAGCAGAAG21770.15028987928484938No Hit
CTCCTGGGTCCCTGCCCGATTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC21630.14932338488430372No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21130.14587161916806923No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21020.14511223071049764No Hit
GTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG20680.1427650300234582No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGACCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC19870.13717316956315834No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19830.1368970283058596No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19610.1353782513907164No Hit
GTGTACTACTGTGCGACTTGGGGGGGGGATTGGTTCGCCCCCTGGGGCCA18880.13033867344501407No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18600.12840568464392274No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18400.12702497835742896No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17970.1240564598414673No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17820.12302093012659696No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17390.12005241161063529No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16860.11639353995142675No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16630.11480572772195889No Hit
CCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCC16560.11432248052168606No Hit
CCCAAGGACCACTATAGGACAGCAAGCAGTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGG15920.10990422040490593No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15850.10942097320463309No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15750.1087306200613862No Hit
GAAGAAACACTCCTGGGTCCCTGCCCGATTCTCAGGCTCCCTCCTTGGGG15680.10824737286111337No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15430.10652149000299613No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15270.1054169249738011No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14750.10182708862891725No Hit
GTGGTGACTCAGGAGACCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC14550.10044638234242345No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG150.002520095367.835775
TCTGGAA14900.044.54633370
GTGGTAT3700.044.0274281
GGTATCA12800.042.9525381
AATGGTA5950.042.14224670
TGGTATC4250.039.1161352
CGTACCC350.001466763638.7566073
GTATCAA31050.035.7412641
GCCTATT850.002521094434.7053870
AGTACAC12950.031.1624093
TATCAAC36350.030.4271972
ATCAACG36600.030.1131423
TCAACGC37150.029.7606624
CCGTGTC13300.029.32648312
CAGCCGT13350.029.2186649
GTAGTAC13700.028.9833811
CAACGCA38350.028.8324175
TAGTACA14050.028.7355422
AACGCAG39100.028.193596
GCCGTGT14100.028.14560311
Produced by FastQC (version 0.11.5)