FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687283
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23620.3436721117792816No Hit
CCATAGATCAGGAGATTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT22650.3295585661219614No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22210.3231565454114244No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19110.2780513994962774No Hit
GATCTATGGTGCGTCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAAATTTCA17950.26117334489577076No Hit
GAGTTACATTACCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTT17160.24967880771094292No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15320.22290672110324275No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15170.22072421404283243No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14970.21781420462895196No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGAAA14930.21723220274617588No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14290.20792017262175844No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGGTGCAGACTGCTGATGGTGAGAGTGAAATTTGTC13870.2018091528526095No Hit
GTCTGCACCCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTACATT13500.19642563543693062No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13090.19046011613847572No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG12840.18682260437112513No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12490.18173008789683434No Hit
ACATTACCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACT11870.17270905871380496No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11730.17067205212408862No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC11540.16790754318090217No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11450.166598038944656No Hit
GTGATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCCTCAGGGAGA11270.16397903047216358No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11070.16106902105828313No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAC10940.15917751493926083No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10930.1590320144685668No Hit
ACTTACTACTGTCAACAGAGTTACATTACCCTCACTTTCGGCCCTGGGAC10690.15554000317191025No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10640.15481250081844014No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10610.15437599940635807No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10340.15044748669761945No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC10330.15030198622692545No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10220.1487014810492912No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10200.14841048010790314No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10010.14564597116471673No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT9710.14128095704389604No Hit
GGGTGCAGACTGCTGATGGTGAGAGTGAAATTTGTCCCAGATCCACTGCC9650.1404079542197319No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9090.13225992786086663No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9050.13167792597809055No Hit
GGGTAATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGGTGC8860.1289134170349041No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8790.12789491374004594No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8740.12716741138657583No Hit
GTAGTAAGTTGCAAAATCCTCAGGGTGCAGACTGCTGATGGTGAGAGTGAAATTTGTCCCAGATCCACTGCCA8470.12323889867783722No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8130.11829188267424046No Hit
GTCCATGAGTGGTTATTTCTGGACCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGCAATATTT8060.1172733793793823No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7980.1161093756138301No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7800.1134903671413377No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7770.11305386572925563No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7570.11014385631537518No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7500.10912535302051703No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7350.10694284596010667No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA7340.10679734548941265No Hit
GATTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGGTGCTA7210.10490583937039036No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7150.10403283654622623No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG7060.10272333230998001No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7010.10199582995650991No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT6930.10083182619095774No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG150.002433565468.4323354
GTATACA150.002433565468.4323354
ACGAACA200.006565766453.2618962
TAGACGA200.007602056551.324255
ACGAGAC405.23841E-542.78577422
ATACCAT504.0649065E-641.05946
CTAGTCA3700.040.6954270
GTATCAA17150.040.1046681
GTGGTAT1300.039.4830671
TTGAACG350.001403782639.10134510
AATGGTA3600.038.023570
TATCAAC19150.035.3775522
TCAACGC19150.035.198884
ATCAACG19200.035.1072123
CAACGCA19550.034.3036775
CCGCATT807.596827E-834.2286221
AACGCAG19550.034.128666
ACGCAGA20800.032.2398157
CACTGAT5950.032.20813870
GTTCGAC3250.031.96087345
Produced by FastQC (version 0.11.5)