FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687283
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25850.3761187167440487No Hit
CCATAGATCAGGAGATTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT20540.2988579668055226No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18380.26742986513561373No Hit
GATCTATGGTGCGTCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAAATTTCA18320.2665568623114496No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17620.256371829362868No Hit
GAGTTACATTACCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTT17360.2525888171248234No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16560.2409487794693016No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16330.23760226864333908No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15870.23090924699141402No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14000.20370065897163178No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGGTGCAGACTGCTGATGGTGAGAGTGAAATTTGTC13460.19584363355415454No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGAAA12820.1865316034297371No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12670.18434909636932675No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12540.18245759025030447No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11770.17125405400686472No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11350.16514303423771576No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAC11330.1648520332963277No Hit
ACATTACCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACT11140.16208752435314128No Hit
GGGTGCAGACTGCTGATGGTGAGAGTGAAATTTGTCCCAGATCCACTGCC10940.15917751493926083No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10770.15670400693746242No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10710.1558310041132983No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10620.1545214998770521No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10380.15102948858039555No Hit
GTGATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCCTCAGGGAGA10350.15059298716831349No Hit
GTCTGCACCCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTACATT10300.14986548481484338No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10100.1469554754009629No Hit
ACTTACTACTGTCAACAGAGTTACATTACCCTCACTTTCGGCCCTGGGAC10100.1469554754009629No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC10080.14666447445957487No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC10030.14593697210610476No Hit
GTCCATGAGTGGTTATTTCTGGACCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGCAATATTT9910.1441909664577765No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9560.1390984499834857No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9350.1360429400989112No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8940.1300774208004563No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG8920.12978641985906825No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT8920.12978641985906825No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8770.1276039127986579No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8580.12483940385547146No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8560.12454840291408344No Hit
GTAGTAAGTTGCAAAATCCTCAGGGTGCAGACTGCTGATGGTGAGAGTGAAATTTGTCCCAGATCCACTGCCA8560.12454840291408344No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8290.12061989020534482No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8000.11640037655521816No Hit
GGGTAATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGGTGC7910.11509087231897194No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7820.11378136808272575No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7790.11334486667064368No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7660.11145336055162137No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA7290.10606984313594255No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7220.10505133984108439No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7010.10199582995650991No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6980.10155932854442784No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAAATTTCACT6970.10141382807373382No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC6950.10112282713234577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGC200.007071688352.2715651
AACGTTT450.00881592149.42999670
GTATCAA17550.039.1235771
TGTTAGA6750.036.24866570
GTGGTAT1650.035.1952931
CACTGAT5250.033.8948570
TATCAAC20350.033.3901672
ATCAACG20350.033.3901673
TCAACGC20400.033.140954
CAACGCA20700.032.830385
AACGCAG21150.032.4571536
ACGCAGA22300.030.7833567
AATGGTA3650.030.47054570
CGCAGAG22800.030.4078678
CCAGAGT5150.030.2338870
AGAGTAC23100.030.16080711
GCAGAGT23100.030.012969
GTATTGG605.7431596E-428.466781
ATGGCAA2404.2211468E-827.8043770
ACCGGGA500.0081327827.3161772
Produced by FastQC (version 0.11.5)