FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1279874
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48520.37909981763829875No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43290.3382364201476083No Hit
CTCCCAGACTGCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCA37070.2896378862294257No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34770.2716673672564643No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34550.26994844805035495No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAATAACTGCTA29190.2280693255742362No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28270.22088111798505167No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGCGCCTGAAGATT28120.2197091276172498No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT27400.21408357385180105No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26890.2100988066012748No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26550.207442295100924No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25950.20275433362971668No Hit
CTCCTGCAGGGCCAGTCAGAGTTTTAGCAGTTATTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGA25410.1985351683056301No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25200.19689438179070753No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24800.19376907414323596No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT24370.19040936842220405No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23770.18572140695099676No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22880.17876759743537254No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22820.17829880128825182No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21910.17118872639025404No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21490.16790715336040893No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT21390.16712582644854101No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21230.1658757033895524No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21160.1653287745512449No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20980.1639223861098827No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGAGTTGCTACGGTGCTGACAGTAATAAA20120.15720297466781885No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19250.15040543053456826No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT19110.1493115728579532No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTTTTAGCAGTTATTTAGCCTGGTA19050.14884277671083246No Hit
GTTTATTACTGTCAGCACCGTAGCAACTCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCA18790.14681132673997596No Hit
GACTTCACTCTCACCATCAGCAGCCTAGCGCCTGAAGATTTTGCAGTTTA18190.14212336526876865No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17920.14001378260672534No Hit
GTAATAAACTGCAAAATCTTCAGGCGCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17840.13938872107723102No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGAGTTGCTACGGTG17350.13556021920907838No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16490.12884080776701456No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA16390.12805948085514668No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15730.12290272323681863No Hit
GTATTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC15670.1224339270896979No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15480.1209494059571489No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15240.11907422136866598No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15210.1188398232951056No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT15050.11758970023611698No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14010.10946390035269098No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13560.10594792924928546No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG13520.10563539848453833No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12910.10086930432214421No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12900.10079117163095741No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12860.10047864086621028No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12820.10016611010146312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12850.045.4190141
TCGTATG1550.040.04053540
CCTAACG603.3227298E-739.5767752
ACGAGAC2950.036.813622
CACGAGA3100.036.12563721
GTATCAA35200.035.8601951
CGTATGC1750.035.52796641
CGAGACA3250.035.51082223
CTAACGC601.5070429E-533.9229473
TCTCCGA3050.033.36161811
ATCAACG39350.031.8970553
TCAACGC39400.031.8578194
GTCGTAT652.361128E-531.76779639
TATCAAC39250.031.7190382
CAACGCA39700.031.6170795
CGGGATT652.6054053E-531.3134917
AACGCAG40550.031.037996
ATCTCCG3500.031.0116310
CCCACGA3550.030.58440819
CTTATAC3750.029.8603611
Produced by FastQC (version 0.11.5)