FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1279874
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49050.3832408502711986No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37180.2904973458324804No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35140.2745582768303755No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33580.26236957700523644No Hit
CTCCCAGACTGCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCA33030.25807227898996304No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAATAACTGCTA28710.22431895639727037No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27610.2157243603667236No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27360.2137710430870539No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27210.21259905271925206No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGCGCCTGAAGATT26660.20830175470397866No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT25540.19955089329105835No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24940.194862931819851No Hit
CTCCTGCAGGGCCAGTCAGAGTTTTAGCAGTTATTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGA24390.19056563380457764No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24050.18790912230422682No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23970.1872840607747325No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23910.18681526462761178No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22410.1750953609495935No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22310.17431403403772558No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21780.17017300140482577No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT21450.16759462259566177No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21140.16517250916887133No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20960.1637661207275091No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGAGTTGCTACGGTGCTGACAGTAATAAA19960.15595285160883024No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19840.1550152593145888No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19760.15439019778509447No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTTTTAGCAGTTATTTAGCCTGGTA19740.1542339324027209No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT19730.15415579971153412No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19430.15181181897593043No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19350.15118675744643614No Hit
GTTTATTACTGTCAGCACCGTAGCAACTCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCA18960.14813958249015138No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT18490.1444673460043723No Hit
GACTTCACTCTCACCATCAGCAGCCTAGCGCCTGAAGATTTTGCAGTTTA18060.14110764028334039No Hit
GTAATAAACTGCAAAATCTTCAGGCGCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17970.1404044460626593No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17760.13876365954773673No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17480.13657594419450664No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGAGTTGCTACGGTG16940.13235677887042005No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15750.12305898861919219No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA15130.1182147617656113No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15070.11774596561849057No Hit
GTATTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC14430.11274547338253608No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14340.11204227916185501No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14320.11188601377948142No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14260.11141721763236068No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14180.11079215610286638No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT13960.10907323689675703No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13880.10844817536726271No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG13500.10547913310216474No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT13300.10391647927842898No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13150.10274448891062714No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13150.10274448891062714No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13110.10243195814587998No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13010.10165063123401209No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTATTGACACAGTC12980.10141623316045174No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12810.10008797741027634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16100.041.504121
TACGTAT800.002517411334.7153970
TAGTACG502.0680165E-433.88784
GTATCAA38850.033.1774861
GCGGCAT850.00338845132.67330670
TATCAAC41000.031.003422
ATCAACG40950.030.8648623
TCAACGC41350.030.4867274
AACGCAG42100.030.2655876
CAACGCA41900.030.0865425
ACGCAGA44950.028.3466347
CGCAGAG45000.028.089228
TAACGGC1103.943387E-828.0143236
GCGATTC854.972604E-627.9918327
TTAACGG1103.981404E-827.9866935
AGAGTAC45600.027.86607711
AATGGTA10800.027.64373670
GCAGAGT46350.027.1979739
CGAGACT500.008441281527.1102394
CCGAAAC908.61661E-526.27400668
Produced by FastQC (version 0.11.5)