FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913404
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65040.3399177591350284No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50100.26183701925991587No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47790.24976429441978795No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46430.24265654299876033No Hit
GTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT46020.24051376499683286No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA44300.2315245499643567No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41780.21835430468421727No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG39200.20487048213550302No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37090.193843014857291No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35720.18668300055816753No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33660.17591684767043447No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT31320.16368733419601925No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30740.16065608726646333No Hit
GTCTCCATCCTCCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTT29200.1526076040397114No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29000.1515623464777956No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28440.14863562530443125No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC28110.14691095032727014No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT27200.1421550284205531No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26800.14006451329672145No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26700.13954188451576352No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26030.13604027168334548No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25990.13583122017096233No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25950.13562216865857915No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC25720.13442012246237597No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG25470.13311355050998117No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25150.13144113841091581No Hit
CAGTTACAATAATCCCATCACCTTTGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT25050.13091850962995794No Hit
CCTTTGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA24800.12961193767756313No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24730.12924609753089258No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGTTATTTAAATTGGTATCAGCAGAAA24660.12888025738422204No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23850.12464696425846292No Hit
GGATTATTGTAACTGTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTG23720.12396754684321763No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACACAGTTACAATAAT23690.12381075820893025No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT23370.12213834610986492No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23120.12083177415747014No Hit
ACTTACTACTGTCAACACAGTTACAATAATCCCATCACCTTTGGCCAAGGGACACGACTGGAGATTAAACGAA22980.12010009386412906No Hit
CAATAATCCCATCACCTTTGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCAT22120.11560548634789097No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21980.1148738060545499No Hit
GGCCAAAGGTGATGGGATTATTGTAACTGTGTTGACAGTAGTAAGTTGCA21580.11278329093071823No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT21440.11205161063737716No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA20490.10708663721827696No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAACTGCTA20470.10698211146208537No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC20130.10520517360682848No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCAAAGGTG19960.1043167046792No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19530.10206940092108095No Hit
CTACATAGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG19470.10175582365250621No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19330.10102414335916514No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGCG200.006241011453.95315658
TACGTAT1759.094947E-1139.40894370
GTATCAA52000.035.587021
CGCGAAC400.00270224534.2209537
ATCAACG57700.031.8828983
GTGGTAT5550.031.5815181
TCAACGC58600.031.3283624
TATCAAC58800.031.286452
CAACGCA59200.031.066125
AACGCAG59750.030.8920946
ACGCAGA63950.028.7578757
TGTTAGA19750.028.63383770
CGCAGAG65050.028.3751358
AGAGTAC65800.027.94933511
GCAGAGT67050.027.5287519
CGGGGTC3950.027.31138217
AATGGTA13150.026.74713170
TGGTACG1501.0279109E-825.3503567
CAGAGTA74800.024.76656710
CGTGCGT1400.001337535624.63058970
Produced by FastQC (version 0.11.5)