FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353501
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47190.3486513862937671No Hit
GAATTAGCTCTCACGATTAGCAGCCTGCAGCCTGAAGACTTTGCAACTTATTACTGTCTACAGCTTAGTAGTTAC44470.32855535385640644No Hit
CTACTAAGCTGTAGACAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTAATCGTGAGAGCTAATTCTGTC42620.3148870965001134No Hit
GCTTAGTAGTTACCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT41150.30402637308727515No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39340.2906536456197668No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33300.2460286324132749No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30290.22379000828222514No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29390.21714058578456907No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAA28900.21352034464695632No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG27370.20221632640094098No Hit
GATCTATGCTGCTTCCGCTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTAG27270.20147750167897915No Hit
GTCTACAGCTTAGTAGTTACCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCAC25650.18950854118319824No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25470.188178656683667No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25080.18529724026801606No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24670.18226805890797274No Hit
ACTTATTACTGTCTACAGCTTAGTAGTTACCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA24180.17864781777035998No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24000.17731793327082876No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23770.17561863641031666No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC23460.1733282797722351No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22040.16283696872037776No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21990.16246755635939686No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20680.15278895250169744No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20520.15160683294655858No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20450.15108965564118534No Hit
GTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTAATCGTGAGAGCTAATTCTGTCCCAGATCCACTGCCG20350.15035083091922355No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20280.14983365361385032No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTT19970.14754329697576876No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19940.14732164955918023No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT19840.14658282483721843No Hit
GTCTCCTTCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT19340.14288870122740951No Hit
GCATAGATCAGGAGGTAAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC19260.14229764144984008No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19220.14200211156105538No Hit
GCTGTAGACAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTAATCGTGAGAGCTAATTCTGTCCCAGATC19160.1415588167278783No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18700.13816022300685407No Hit
GTCTTCAGGCTGCAGGCTGCTAATCGTGAGAGCTAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC18400.13594374884096871No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18340.13550045400779165No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC18290.13513104164681075No Hit
GTAATAGACGGCCGTGTCCTCGGTTTTCAGGCTACTCATTTGCAGATACA18180.1343183344526528No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17940.1325451551199445No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17920.13239739017555213No Hit
CCTTCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG16970.12537855531691516No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16570.12242325642906801No Hit
GCCTGAAGACTTTGCAACTTATTACTGTCTACAGCTTAGTAGTTACCCTC16380.12101948945734065No Hit
GATCAGGAGGTAAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT15510.1145917143762731No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCTTCCTTC15010.11089759076646415No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14750.10897664648936352No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14380.1062429950181049No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14260.10535640535175075No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13850.10232722399170742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)