FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353501
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47100.3479864440440014No Hit
CTACTAAGCTGTAGACAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTAATCGTGAGAGCTAATTCTGTC42350.3128922697508166No Hit
GAATTAGCTCTCACGATTAGCAGCCTGCAGCCTGAAGACTTTGCAACTTATTACTGTCTACAGCTTAGTAGTTAC41370.3056517874755911No Hit
GCTTAGTAGTTACCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT39820.2942000042851834No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33920.250609345689438No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33080.24440321802495896No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30480.22519377525395254No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28090.20753586439906582No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26320.1944586668203422No Hit
GTCTACAGCTTAGTAGTTACCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCAC25860.191060073099318No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAA25350.18729206701731288No Hit
GATCTATGCTGCTTCCGCTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTAG25190.18610994746217402No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24350.179903819797695No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23790.175766401354709No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23460.1733282797722351No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG23230.17162898291172302No Hit
ACTTATTACTGTCTACAGCTTAGTAGTTACCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA22540.16653109233018668No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22080.16313249860916246No Hit
GTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTAATCGTGAGAGCTAATTCTGTCCCAGATCCACTGCCG21760.16076825949888476No Hit
GTCTTCAGGCTGCAGGCTGCTAATCGTGAGAGCTAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC21590.1595122574715497No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20900.1544143668900134No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTT20360.15042471339141972No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20330.15020306597483118No Hit
GCTGTAGACAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTAATCGTGAGAGCTAATTCTGTCCCAGATC20330.15020306597483118No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20080.14835600416992673No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20060.14820823922553436No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19930.14724776708698406No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19760.145991765059649No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC19690.14547458775427574No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19450.14370140842156748No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19080.14096775695030886No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18900.13963787245077763No Hit
GTCTCCTTCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT18760.13860351784003117No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18490.13660869109073434No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18340.13550045400779165No Hit
GTAATAGACGGCCGTGTCCTCGGTTTTCAGGCTACTCATTTGCAGATACA18250.13483551175802602No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18190.13439221692484896No Hit
GCATAGATCAGGAGGTAAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC18170.13424445198045662No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC18140.13402280456386806No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18120.13387503961947572No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT18020.13313621489751393No Hit
AGCTAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAAGCGGAAGCAGCATAGAT16650.12301431620663746No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15860.11717760090313935No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15860.11717760090313935No Hit
CCTTCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG15790.1166604235977661No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTAGCT14900.1100848835723062No Hit
GCCTGAAGACTTTGCAACTTATTACTGTCTACAGCTTAGTAGTTACCCTC14710.10868111660057879No Hit
GATCAGGAGGTAAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT14400.10639075996249725No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCTTCCTTC14100.10417428579661189No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13910.1027705188248845No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13790.10188392915853035No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13570.10025851477021441No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT200.0075346951.4426333
GTATCAA36700.037.6641161
ATCAACG39450.034.1431543
TATCAAC39950.033.8978162
TCAACGC39750.033.8854684
TTACAGT10550.033.3993770
AACGCAG40550.033.216956
CAACGCA40600.033.0920525
GTGGTAT3200.033.0636331
ACGCAGA42700.031.3847167
CTAAACG450.004315056431.10172752
CGCAGAG43500.030.7291368
CGGTACC802.6311118E-630.37620745
AGAGTAC43950.030.1817411
CAGAGTA44400.030.10624710
GCAGAGT44900.029.8469359
TGTTAGA13400.029.38939370
ATGGCAA5500.028.89253870
AATGGTA10550.028.81514270
ATACGTA2850.027.51924511
Produced by FastQC (version 0.11.5)