FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences958113
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36920.38534076878197043No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34550.3606046468422827No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27930.29151050032720566No Hit
CATTAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCGACACCCTGCAGCCTGAAGATTTTG27090.2827432672346581No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26860.2803427153164606No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24380.25445850332893927No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21830.22784368858370568No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21230.22158137923188598No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20760.21667590357296063No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGCACATTAACAGTTTTTTAGGCTGGCATCAGAAAAAA20560.21458846712235405No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20180.21062233786620158No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20080.2095786196408983No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19500.20352505393413928No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT18750.19569716724436467No Hit
ACCTTAATGAGACCCCATCTTGCAATGAGGAGACAGAATGGATTATGGGCTTAGGGGCTTCCCCCGGTTTTTT18300.19100043523049995No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18180.18974797336013602No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17560.18327692036325569No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17170.1792064192845729No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16590.17315285357781388No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16310.1702304425469647No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16190.16897798067660078No Hit
GTGCAGCCACAGTTCGCTTGATATCCACTTTTGTCCCTGGGCCGAAAGTG16170.16876923703154012No Hit
ACTTGATGCTTACCCCTTCACTTTCGGCCCAGGGACAAAAGTGGATATCAAGCGAACTGTGGCTGCACCAT16090.1679342624512975No Hit
GAATTCACTCTCACAATCGACACCCTGCAGCCTGAAGATTTTGCAACTTATTTTTGTCAACAACTTGATGCTTAC15700.1638637613726147No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATATCCACTTTTGTCC15040.15697522108561307No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14980.1563489901504311No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14700.15342657911958194No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14440.15071291173379342No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT14150.14768612888041388No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13840.14445060238197374No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13280.13860578032027537No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12750.133074073726168No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12440.12983854722772784No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12020.12545493068145408No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11960.1248286997462721No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11740.12253251965060488No Hit
CTTTTGTCCCTGGGCCGAAAGTGAAGGGGTAAGCATCAAGTTGTTGACAAAAATAAGTTGCAAAATCTTCAGG11710.1222194041830139No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11600.12107131413518031No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11570.12075819866758933No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11370.11867076221698275No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11050.11533086389601227No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11030.11512212025095162No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11000.11480900478336063No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT10910.11386965838058767No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10740.1120953373975721No Hit
TTGCAAGATGGGGTCTCATTAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCGACACCCTG10650.11115599099479916No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10640.11105161917226883No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10540.11000790094696554No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC10470.10927729818925325No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10450.10906855454419259No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10330.10781609267382866No Hit
GTGAAGGGGTAAGCATCAAGTTGTTGACAAAAATAAGTTGCAAAATCTTC10190.10635488715840408No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10050.10489368164297948No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10040.10478930982044915No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9870.10301498883743358No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9680.10103192420935735No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9600.10019694962911471No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGCG200.007775528451.03377
GGTATCA11400.047.7533231
CGGTAGT306.050611E-446.3947746
ATGCGGT306.1757874E-446.20241543
TCGTATG1900.043.57383740
CGTATGC1950.042.53082741
GTATCAA30550.039.759921
GCGGTAG350.001299044939.7245445
GTGGTAT2350.037.643841
ACGAGAC2700.036.55982222
ATCTCCG2650.035.9463910
ATGCCGT2150.033.89640444
ATCTCGT2050.033.52331537
GCCGTCT2200.033.21443646
TATCAAC38050.031.8317952
ATCAACG38450.031.5891323
TCAACGC38850.031.1763134
CTCTTAT1750.031.107881
CGAGCCC3100.030.72836715
CAACGCA39700.030.6802125
Produced by FastQC (version 0.11.5)