FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences958113
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36570.38168775499340896No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29770.310714915672786No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27970.29192798761732697No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27460.28660502466828025No Hit
CATTAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCGACACCCTGCAGCCTGAAGATTTTG24500.2557109651993032No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24190.2524754387008631No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20790.2169890190405516No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20780.21688464721802128No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20760.21667590357296063No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19840.20707369590017044No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18990.19820209098509256No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18480.19287912803604587No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18170.18964360153760568No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18030.1881823960221811No Hit
ACCTTAATGAGACCCCATCTTGCAATGAGGAGACAGAATGGATTATGGGCTTAGGGGCTTCCCCCGGTTTTTT18010.18797365237712044No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGCACATTAACAGTTTTTTAGGCTGGCATCAGAAAAAA17790.18567747228145323No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17280.18035450933240652No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT17020.17764084194661797No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16990.177327726479027No Hit
GAATTCACTCTCACAATCGACACCCTGCAGCCTGAAGATTTTGCAACTTATTTTTGTCAACAACTTGATGCTTAC16250.16960421161178274No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16220.16929109614419174No Hit
GTGCAGCCACAGTTCGCTTGATATCCACTTTTGTCCCTGGGCCGAAAGTG15760.16448999230779668No Hit
ACTTGATGCTTACCCCTTCACTTTCGGCCCAGGGACAAAAGTGGATATCAAGCGAACTGTGGCTGCACCAT15160.158227682955977No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14960.15614024650537045No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14940.1559315028603098No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATATCCACTTTTGTCC14820.15467904098994587No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14230.14852110346065653No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13990.14601617971992867No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13700.14298939686654913No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13600.14194567864124588No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT13290.1387101521428057No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13130.13704020298232047No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12750.133074073726168No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12300.12837734171230325No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12270.12806422624471225No Hit
CTTTTGTCCCTGGGCCGAAAGTGAAGGGGTAAGCATCAAGTTGTTGACAAAAATAAGTTGCAAAATCTTCAGG11920.12441121245615079No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11400.11898387768457375No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11210.1170008130564975No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11030.11512212025095162No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10990.1147046329608303No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10650.11115599099479916No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10590.11052976005961719No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10500.10959041365684423No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10450.10906855454419259No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10350.10802483631888933No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10340.107920464496359No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10330.10781609267382866No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10170.10614614351334342No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10140.10583302804575243No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT10090.10531116893310079No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9760.10186689878959998No Hit
GCATCAAGTTGTTGACAAAAATAAGTTGCAAAATCTTCAGGCTGCAGGGTGTCGATTGTGAGAGTGAATTCTGTC9720.10144941149947866No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGT200.00781871150.9622515
GGTATCA12600.045.2997781
TCTAGTG3150.039.28373770
CTAACGC350.001456601238.8101433
GTGGTAT3350.037.524441
GTATCAA30100.034.7649461
TTCGATC400.002620856334.43329639
TGGTATC3800.033.0721242
CGCATCA450.00430687631.11283350
AGCGCGT450.00495069830.23932523
TCAACGC35250.029.5801624
ATCAACG35500.029.5585693
TATCAAC35700.029.3991152
GTCTAGT3050.029.081269
CAACGCA36400.028.832245
AATGGTA4300.028.77762270
AACGCAG36500.028.7532466
GGACTCG2550.027.970516
ACGCAGA37850.027.7277037
CGCAGAG38300.027.49068
Produced by FastQC (version 0.11.5)