FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004692615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443569
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTACAGTATCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATTCACCGAACTGTGGCTGCACCAT51580.35730886434940073No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45640.31616084856352555No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42190.2922617484858708No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33830.23434972626871314No Hit
CTGTAACTCTGTTGACAGTAGTAAATTGCAAAGTCGTCTGGGTGCAGACTGCTGATGGTGAGAGTGAAAT32720.22666045059155465No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30440.21086626271414805No Hit
ACAGTATCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATTCACCGA29230.20248425949850685No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27740.1921626191751139No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27400.18980734554427256No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25820.17886225043624518No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24940.172766248097597No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGT24580.1702724289590591No Hit
GTATCCATCCTCCCTGTCTGCGTCTGTGGGAGACAGAATCACTATCTCTT23910.1656311544512247No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23200.1607127889279972No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22480.15572515065092143No Hit
AGGTTGGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGAGACAGATTTCACTCTCACCATCAGCAGT22140.1533698770200801No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20920.1449186010505906No Hit
GGATACTGTAACTCTGTTGACAGTAGTAAATTGCAAAGTCGTCTGGGTGCAGACTGCTGATGGTGAGAGTGAAAT20700.14339460046592856No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20150.13958459900427345No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20140.13951532625042515No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19870.13764496189652176No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19690.1363980523272528No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18640.12912441317318396No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18470.1279467763577633No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGTCTCAGGGGCCAGTGGTG18350.127115503311584No Hit
GTGCAGCCACAGTTCGGTGAATCTCCACCTTGGTCCCTTGGCCGAACGTC18240.12635350301925297No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18200.12607641200385988No Hit
GTCTGCACCCAGACGACTTTGCAATTTACTACTGTCAACAGAGTTACAGTATCCCGTGGACGTTCGGCCAAGGGA17720.1227513198191427No Hit
CCCCTAGACTCCTGATGTTCGCAGCAGCGAGGTTGGAGAGTGGGGTCCCA17550.12157368300372202No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTGAATCTCCACCTTGGTCCCTTGGCCGAACGTCCACGGGATA17550.12157368300372202No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17310.11991113691136343No Hit
CTCCTGATGTTCGCAGCAGCGAGGTTGGAGAGTGGGGTCCCATCAAGGTT17200.11914913661903243No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT16950.11741731777282555No Hit
AGTCAGACCATTAGTAGGTATTTAAATTGGTATCAGCAGAAGCCAGGAGA16690.1156162261727704No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15900.1101436786187567No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15830.10965876934181878No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15290.10591804063401196No Hit
GTCTAGGGGCTTCTCCTGGCTTCTGCTGATACCAATTTAAATACCTACTA15080.10446331280319818No Hit
ATTTACTACTGTCAACAGAGTTACAGTATCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATTCACCGAA15030.10411694903395682No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14660.10155385714157066No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14630.10134603888002582No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)