FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443569
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTACAGTATCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATTCACCGAACTGTGGCTGCACCAT50900.352598317087718No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46270.32052503205596683No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45460.3149139389942566No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36260.25118300545384387No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33750.23379554423792695No Hit
CTGTAACTCTGTTGACAGTAGTAAATTGCAAAGTCGTCTGGGTGCAGACTGCTGATGGTGAGAGTGAAAT33630.23296427119174767No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31410.21758571983743072No Hit
ACAGTATCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATTCACCGA27370.18959952728272775No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26390.18281079740559683No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24840.17207352055911426No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24700.17110370200523842No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24030.166462427497404No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGT23380.16195969849726613No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21630.14983696657381806No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21410.14831296598915603No Hit
GTATCCATCCTCCCTGTCTGCGTCTGTGGGAGACAGAATCACTATCTCTT21330.14775878395836983No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20850.14443369177365267No Hit
AGGTTGGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGAGACAGATTTCACTCTCACCATCAGCAGT20810.14415660075825956No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20770.14387950974286645No Hit
GGATACTGTAACTCTGTTGACAGTAGTAAATTGCAAAGTCGTCTGGGTGCAGACTGCTGATGGTGAGAGTGAAAT20700.14339460046592856No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19690.1363980523272528No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19620.13591314305031488No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19520.13522041551183211No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19130.13251877811174942No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18600.12884732215779085No Hit
GTGCAGCCACAGTTCGGTGAATCTCCACCTTGGTCCCTTGGCCGAACGTC18440.12773895809621846No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18420.12760041258852192No Hit
GTCTGCACCCAGACGACTTTGCAATTTACTACTGTCAACAGAGTTACAGTATCCCGTGGACGTTCGGCCAAGGGA17720.1227513198191427No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17310.11991113691136343No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17240.11942622763442552No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTGAATCTCCACCTTGGTCCCTTGGCCGAACGTCCACGGGATA16980.11762513603437037No Hit
CTCCTGATGTTCGCAGCAGCGAGGTTGGAGAGTGGGGTCCCATCAAGGTT16560.11471568037274282No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGTCTCAGGGGCCAGTGGTG15530.10758058672637055No Hit
CCCCTAGACTCCTGATGTTCGCAGCAGCGAGGTTGGAGAGTGGGGTCCCA15170.10508676758783267No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15130.10480967657243957No Hit
GTCTAGGGGCTTCTCCTGGCTTCTGCTGATACCAATTTAAATACCTACTA15100.10460185831089473No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14860.10293931221853614No Hit
CTCCAACCTCGCTGCTGCGAACATCAGGAGTCTAGGGGCTTCTCCTGGCT14770.10231585743390167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAT1304.3937143E-738.18689770
CCGAAAC952.1664164E-937.7263968
GTATCAA49500.036.176231
TCGGAAT400.002270106735.45797754
GTGGTAT5400.035.0386161
TCTCGCG4600.032.3758570
ATCAACG55850.031.136213
TATCAAC56250.031.098122
TCAACGC56200.030.8768754
CTAACGC553.695E-430.6965123
CAACGCA57650.030.1609155
AACGCAG58200.030.0499216
CCTAACG450.00513304230.017492
CGAAACC1104.1075473E-628.6871969
TGTTAGA16550.028.2817170
AGAGTAC62300.028.23392311
CGCAGAG61900.028.199188
GAGTTTA6450.027.7631551
GCAGAGT63450.027.5635229
ATGGCAA5050.026.68221970
Produced by FastQC (version 0.11.5)