FastQCFastQC Report
Wed 8 May 2024
EGAF00004692619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1484889
Total Bases109.6 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC71570.48198888940520135No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT69400.46737500244125996No Hit
ATCTTATGGTGTTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA65140.43868598932310765No Hit
CCATAAGATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT61840.41646210592172206No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCTAGCCAGAGTGTTT52440.35315771077838143No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC49560.3337623216280813No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48910.3293848900490205No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46850.31551179919845856No Hit
TCTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCATA44530.2998877357162724No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG44260.29806941798343173No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT40530.27294969522974444No Hit
GTTTATTACTGTCAGCAATCTTATGGTGTTCCGCTCACTTTCGGCGGAGG38720.2607602319095906No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37990.2558440395207992No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37930.2554399689135013No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG37910.2553052787110686No Hit
CTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT37400.25187067854903633No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC36940.24877280389308562No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA36710.24722386656511028No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33460.2253367086698063No Hit
CCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCTAGC33310.22432653215156148No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32570.21934299466155383No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC32440.2184675083457417No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC32260.2172552965238479No Hit
GTAATATACAGCCGTGTCGTCGCCTCTCAGGCTGTTCATTTGCAGATACA32080.21604308470195413No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31750.21382069636181558No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC30970.20856777846694263No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA30790.2073555666450489No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG30620.20621069992437144No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTA29780.2005537114222006No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29440.1982639779808457No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA29320.19745583676624984No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC28880.1944926523127318No Hit
GTCTCCAGACTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACT28870.19442530721151546No Hit
AGTCTAGCCAGAGTGTTTTGTCCAGCTTCAACAATATGAACTACTTAGCT28630.1928090247823238No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC26660.17954203984270878No Hit
ACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGATGCTCATT26640.17940734964027613No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCT25560.1721340787089136No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25400.17105655708945247No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG25230.16991169036877504No Hit
GTATATTACTGTGTGAGTCCTCACGGCGGTGGCGATTCTTATTATTTTGA24080.16216700372889825No Hit
AACCAGGACAGCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGAA23460.15799160745348642No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCATCTTAG23330.15711612113767426No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23180.15610594461942948No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC23010.15496107789875202No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22490.1514591326355034No Hit
GTTTTGTCCAGCTTCAACAATATGAACTACTTAGCTTGGTACCAGCAAAA22120.14896736389049955No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21880.14735108146130788No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21870.14728373636009157No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA21420.14425320680535716No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC21300.1434450655907613No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21050.14176143806035332No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC20840.14034719093481063No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA20780.1399431203275127No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20530.1382594927971047No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20270.13650852016548037No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC19940.13428613182534183No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC19870.13381471611682758No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT19800.13334330040831335No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC19790.13327595530709702No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG19750.13300657490223175No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT19450.13098622186574216No Hit
GTAAATGAGCATCTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCTA19450.13098622186574216No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA19430.1308515316633095No Hit
CTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGATGCTCA19340.13024542575236264No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT19300.12997604534749735No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18910.1273495864000609No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18900.12728224129884455No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18530.12479047255384074No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG18460.12431905684532649No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18330.12344357052951434No Hit
ATGGTGTTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA18320.12337622542829801No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGA18270.12303949992221641No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTCTGTCTC18210.12263542931491848No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT18130.12209666850518794No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCC17990.12115383708815947No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17900.12054773117721257No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATCTTATGGTGTTCCGC17880.12041304097477994No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGT17800.11987428016504936No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT17610.11859472324193929No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17570.11832534283707401No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG17450.11751720162247818No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT17270.11630498980058442No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT17080.11502543287747434No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA17070.11495808777625802No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16820.11327446024585001No Hit
CATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTG16780.11300507984098473No Hit
CCCAGAGACAGAGCCAGGGAGTCTGGAGACTGGGTCATCACGATGTCCCC16750.11280304453733579No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16630.11199490332273994No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16360.11017658558989932No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16340.11004189538746667No Hit
GCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCC16220.10923375417287084No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCC16160.10882968356557292No Hit
GTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCATATTGTTGAAGCTG16150.1087623384643566No Hit
CTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCTA15790.10633791482056908No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGC15650.10539508340354059No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA15590.10499101279624268No Hit
GCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGATGCTCATTTACTG15180.10222986364637357No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC15150.1020278283427246No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15090.1016237577354267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)