FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1484889
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62140.4184824589582117No Hit
ATCTTATGGTGTTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT56630.38137530818801946No Hit
CCATAAGATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC56490.38043247677099096No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55490.37369796664935895No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40870.2752394286710993No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCTAGCCAGAGTGTTTTGTCCAGCTTCAACAATATGAACTA40690.27402721684920556No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39500.2660131498044635No Hit
TCTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCATATTGTTGAAGCTGGACAAAACACT37740.2541604119903912No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37420.25200536875146895No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36460.24554023903470226No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36440.24540554883226962No Hit
GTTTATTACTGTCAGCAATCTTATGGTGTTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA35070.23617926996563376No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA33030.22244086931750454No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31470.21193503352775864No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30730.20695149603775098No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG30710.20681680583531833No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29570.19913946429665785No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29270.19711911126016826No Hit
CTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT29170.19644566024800506No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28490.1918661933652953No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC27700.18654593036920605No Hit
GTAATATACAGCCGTGTCGTCGCCTCTCAGGCTGTTCATTTGCAGATACA27070.1823031889925779No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26840.18075425166460254No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26550.17880124372932926No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATCTTATGGTGTT26260.17684823579405598No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26180.17630947498432542No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25710.17314425522715837No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT25440.17132593749431776No Hit
CCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCTAGC25350.1707198315833709No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC24990.16829540793958336No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC24900.1676893020286365No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24810.16708319611768963No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24000.1616282429191677No Hit
GTCTCCAGACTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCTAGCCAGAGTGTTTTGTC22210.14957346980144645No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG21640.1457347990321162No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT21420.14425320680535716No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21300.1434450655907613No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21150.14243488907251653No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20700.13940435951778213No Hit
AGTCTAGCCAGAGTGTTTTGTCCAGCTTCAACAATATGAACTACTTAGCTTGGTACCAGCAAAAACCAGGACAGC20420.13751869668372518No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20070.135161618141154No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20030.1348922377362887No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC19790.13327595530709702No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19650.13233312389006854No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCATCTTAGGAGGCTGTCCTGGTTTTTGCTGGTA19630.1321984336876359No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19630.1321984336876359No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA19600.13199639838398697No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19130.12883117862681992No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19080.12849445312073832No Hit
ACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGATGCTCATT18820.12674348048911402No Hit
GTATATTACTGTGTGAGTCCTCACGGCGGTGGCGATTCTTATTATTTTGA18140.12216401360640426No Hit
AACCAGGACAGCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGAA18040.12149056259424106No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17960.12095180178451048No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC17870.12034569587356361No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA17530.11805596243220874No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAAT17450.11751720162247818No Hit
GTTTTGTCCAGCTTCAACAATATGAACTACTTAGCTTGGTACCAGCAAAA17380.11704578591396395No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17380.11704578591396395No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17230.11603560939571914No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17160.11556419368720491No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC16700.11246631903125419No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT16630.11199490332273994No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATCTTATGGTGTTCCGC16580.11165817781665835No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16560.11152348761422572No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATCTTATGGTGTTCCGCTCACTTT16530.11132145231057675No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16450.11078269150084619No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAAT16230.10930109927408714No Hit
GTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCATATTGTTGAAGCTG16120.10856030316070764No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15650.10539508340354059No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15550.1047216323913774No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15420.10384614607556525No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15240.1026339342536715No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT15110.10175844793785932No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15080.10155641263421038No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTCTGTCTCTGGGCGAGAGGG15080.10155641263421038No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15030.10121968712812877No Hit
ATGGTGTTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA15000.10101765182447982No Hit
GTAAATGAGCATCTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCATATTGTTGAAGCTGGA15000.10101765182447982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT702.2645872E-762.41268570
CATAACG200.004147270759.82123668
ATACCGG200.00791900950.7995641
GGTATCA14400.046.5662651
CGAGAGT750.001451824638.83455770
TGGTATC3750.038.811212
GTATCAA34400.037.1151851
GTGGTAT3950.036.8671951
ATACGTA1155.820766E-1132.38301511
GTGTCGT8300.032.2158114
CGTGTCG8300.032.2125513
GTACGTA852.2474014E-531.99752869
GCCGTGT8600.031.88665411
AGCCGTG8700.031.52014210
CCTAACG553.6436386E-430.7699852
GTAATAT9150.030.7203221
TGTCGTC8500.030.67171715
CCGTGTC8850.030.6023312
AATATAC9150.030.3328173
ACAGCCG9350.030.0530748
Produced by FastQC (version 0.11.5)