FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359738
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCT74650.5490028226025896No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC66420.48847645649382454No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAACAACTTCCAGGAACAGC48470.3564657308981583No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44240.3253567966770069No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT44030.3238123815029072No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTC41560.30564711731230576No Hit
CCCTCGAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCC41400.3044704200368012No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGTCCAG35790.2632124718144231No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCGTCAGGGAGA35520.26122679516200914No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA34620.2546078729872961No Hit
GTTCGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTCGGTCAGCCC33260.24460594614550743No Hit
TCACTGGACTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT28970.21305575044604183No Hit
GATGTACACTGGTACCAACAACTTCCAGGAACAGCCCCCAAGCTCCTCAT28240.20768706912655233No Hit
GGTTATGATGTACACTGGTACCAACAACTTCCAGGAACAGCCCCCAAGCTCCTCATCTCTGGTAACATCAATCGG27450.20187712632874863No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG27350.20114169053155828No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27280.20062688547352503No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27210.20011208041549178No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26970.19834703450223498No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25340.18635943100803243No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTCGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTC24580.18077011894938586No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24560.18062303178994776No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23840.17532789405017732No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT23840.17532789405017732No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23590.17348930455720146No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23570.17334221739776337No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23560.17326867381804437No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGTCCAGTGATGGCCAGGGAGG22050.16216359328047023No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21960.16150170106299888No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21610.1589276757728327No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21580.1587070450336756No Hit
GTCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAAT21520.15826578355536142No Hit
GTCTATTACTGTGCGAGAGCCTTTCCCGAGCATTACGATTTTTGGAGTGGTTCGTCGCGGGAAAACTGGTTCGAC21230.15613301974350943No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAA21200.1559123890043523No Hit
GTCCTATGACAGCAGCCTGAGTGGTTCGAGGGTGTTCGGCGGAGGGACCA20820.153117732975029No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG19660.14458667772762104No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTT19540.14370415477099266No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19520.1435570676115546No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19100.14046823726335514No Hit
GCCTCATCCTCAGCCTGGAGTCCAGTGATGGCCAGGGAGGCTGAGGTGCC18980.13958571430672673No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC18700.1375264940745938No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17820.13105465905931876No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG17720.13031922326212844No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17680.1300250489432523No Hit
GTCTCTGGTGACTCCATCACCAGTTACTACTGGAGCTGGATCCGGCAGCC17520.12884835166774775No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16510.12142045011612532No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16210.11921414272455429No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT15910.11700783533298326No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC15620.11487507152113127No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGACTCCAGGCTGAGGATG15520.11413963572394092No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCGAACCA15180.11163915401349378No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15150.11141852327433667No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15100.11105080537574151No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT14990.11024182599883212No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14980.1101682824191131No Hit
CCTCATCTCTGGTAACATCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGC14900.10957993378136081No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGC14900.10957993378136081No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCGGCTCCCAGATGGGTC14850.10921221588276565No Hit
GCTTGGTCCCTCCGCCGAACACCCTCGAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCAT14810.10891804156388951No Hit
GTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGA14680.10796197502754208No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14680.10796197502754208No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC14650.10774134428838497No Hit
GGCCATCACTGGACTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT14580.10722653923035173No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG14310.10524086257793781No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14250.10479960109962361No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14210.10450542678074747No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14200.10443188320102842No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13990.10288746802692872No Hit
CCCCTGAGGGCCGATTGATGTTACCAGAGATGAGGAGCTTGGGGGCTGTTCCTGGAAGTTGTTGGTACCAGTGTA13890.10215203222973838No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT13870.1020049450703003No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATT13690.1006811606353577No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13690.1006811606353577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGTA3200.048.01145630
TCGTATG2450.047.85294740
ACGCGTA3100.047.2825627
CGTATGC2200.047.0598441
CGCGTAG3300.046.50168628
GCGTAGT3350.045.83295429
GACGCGT3250.045.08202726
TGATGAT11450.044.18759570
CGAGACG3400.043.0661723
AGACGCG3450.042.45920625
GGTATCA12300.042.3641
ACGAGAC3550.042.2010422
ATCTCGT2450.042.0665137
AATCTCG2450.042.0212936
ATGCCGT2150.041.94790644
CACGAGA3850.039.79556321
CCCACGA3850.039.79556319
ATCTCCG3850.039.79263310
CGAGCCC3850.039.79263315
GAGACGC3700.039.57868624
Produced by FastQC (version 0.11.5)