FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359738
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC65620.4825929701163018No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCT57650.4239787370802316No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAACAACTTCCAGGAACAGC46940.3452135632011461No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44130.3245478173000975No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40000.29417431887613643No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG39430.2899823348321515No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36310.26703673795981286No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTC33610.24717997143567363No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCGTCAGGGAGA31830.23408921424568555No Hit
GTTCGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTCGGTCAGCCC31750.23350086560793326No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31430.23114747105692418No Hit
CCCTCGAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCC30380.22342539518642562No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30040.22092491347597845No Hit
GGTTATGATGTACACTGGTACCAACAACTTCCAGGAACAGCCCCCAAGCTCCTCATCTCTGGTAACATCAATCGG29330.21570331931592704No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG28070.20643682827132873No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGTCCAG27650.20334799792312933No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT27120.19945018819802052No Hit
GATGTACACTGGTACCAACAACTTCCAGGAACAGCCCCCAAGCTCCTCAT27010.1986412088211111No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26960.19827349092251595No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26490.19481694267572136No Hit
TCACTGGACTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT25990.19113976368976965No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25510.18760967186325603No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23870.17554852478933441No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTCGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTC23760.17473954541242503No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC23560.17326867381804437No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23290.17128299716563045No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC23110.16995921273068781No Hit
GTCCTATGACAGCAGCCTGAGTGGTTCGAGGGTGTTCGGCGGAGGGACCA23020.1692973205132165No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22360.16444344425176027No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAA21960.16150170106299888No Hit
GTCTATTACTGTGCGAGAGCCTTTCCCGAGCATTACGATTTTTGGAGTGGTTCGTCGCGGGAAAACTGGTTCGAC21580.1587070450336756No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21560.15855995787423754No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT21450.15775097849732816No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21190.15583884542463328No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG20890.15363253803306226No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20090.1477490516555395No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC19780.14546920068424948No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19470.1431893497129594No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT18230.1340699458277992No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17530.12892189524746678No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTT17420.1281129158705574No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG17280.12708330575449095No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17140.12605369563842447No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG16900.12428864972516764No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC16880.12414156256572957No Hit
GTCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAAT16840.12384738824685343No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16660.12252360381191084No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16090.11833161976792589No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGTCCAGTGATGGCCAGGGAGG15980.1175226403910165No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15880.11678720459382616No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15820.11634594311551195No Hit
CCTCATCTCTGGTAACATCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGC15790.11612531237635486No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15790.11612531237635486No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGACTCCAGGCTGAGGATG15650.11509570226028837No Hit
GTCTCTGGTGACTCCATCACCAGTTACTACTGGAGCTGGATCCGGCAGCC15500.11399254856450287No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15480.1138454614050648No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15100.11105080537574151No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCGGCTCCCAGATGGGTC15000.11031536957855116No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG14580.10722653923035173No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC14430.10612338553456621No Hit
GGCCATCACTGGACTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT14310.10524086257793781No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14190.1043583396213094No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14030.10318164234580486No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT13990.10288746802692872No Hit
GCTTGGTCCCTCCGCCGAACACCCTCGAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCAT13740.10104887853395286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)