FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644226
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT51320.31212254276480245No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA44580.2711306109987313No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42990.2614604075108896No Hit
GTATGGGATGACAGCCTGACTACTTATGTCTTCGGAACTGGGACCGAGGT40800.24814107063140955No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG36750.2235094202378505No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC35590.21645442901401632No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG35350.214994775657361No Hit
ATCCCATACTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG34960.21262283895279602No Hit
CATTAATACTGGTGGTACTACCATATTCTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGT32440.19729647870791486No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32050.1949245420033499No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC29860.18160520512386982No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG29760.18099701622526346No Hit
GTATTTATACCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAG29330.1783818039612559No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA29230.17777361506264955No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC28820.17528004057836333No Hit
GCCTTGGGCTGACCTCGGACGGAGACCTCGGTCCCAGTTCCGAAGACATAAGTAGTCAGGCTGTCATCCCATACT27290.16597475042968546No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT24570.1494320123875915No Hit
GTATTAATGTGTGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGAC24030.14614779233511696No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23140.14073491113752004No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTCGGACGGAGACCTCGGTC23130.1406740922476594No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22970.13970099000988914No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG22540.13708577774588165No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGGTTCCTGACCGATTCTCTGG22280.135504486609505No Hit
GGACAGAGGGTCACCATCTCCTGCTCTGGAGGCAGCTCCAACATTGGGAGTTATTATGTATCCTGGTACCAGCAG22270.1354436677196444No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21470.13057815653079322No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT21450.13045651875107195No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG20950.12741557425803995No Hit
GATTATTACTGCGGAGTATGGGATGACAGCCTGACTACTTATGTCTTCGG20810.126564109799991No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20410.12413135420556543No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG20390.12400971642584414No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19610.11926584301671425No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19150.11646817408312482No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18650.11342722959009285No Hit
ATAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA18020.10959563952887255No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCACCATCTCCTGCTCTGG17830.10844008062152041No Hit
GTATGGGATGACAGCCTGACTACTTATGTCTTCGGAACTGGGACCGAGGTCTCCGTCCGAGGTCAGCCCAAGGC17820.10837926173165977No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG17790.10819680506207784No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17520.10655469503584059No Hit
ACCATATTCTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGTCAAGAATTCACTGTATCTG17370.10564241168793098No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCA17270.10503422278932459No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAG17250.10491258500960329No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCACCATCTCCTG17210.10466930945016076No Hit
ACTTATGTCTTCGGAACTGGGACCGAGGTCTCCGTCCGAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC17160.10436521500085753No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC16800.10217573496587452No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16790.10211491607601388No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGGCAGACTTGGAGCC16690.1015067271774075No Hit
CCTCAGGGGTTCCTGACCGATTCTCTGGCTCCAAGTCTGCCACGTCAGCCACCCTGGGCATCACCGGACTCCAGA16630.10114181383824364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11650.049.9576341
CTACGCT3450.043.44719728
ACTACGC3450.042.45071427
ACGAGAC3650.041.9589822
CGAGACT3600.041.598923
TACGCTG3700.041.4449129
GTATCAA31500.039.9781651
CACGAGA4100.039.84276221
CTTATAC3950.039.636241
AGACTAC3800.039.41547825
GACTACG3950.037.9267726
TATACAC4250.037.6369323
TGCATCG2300.037.17144834
GAGACTA4100.036.5303124
GGTACGG502.0168361E-434.0333943
TAGACCC400.00277635634.032365
CGAGCCC4600.034.02925515
TATCAAC37150.033.8043672
TGGTATC2850.033.4363172
GCATCGG1950.033.34838535
Produced by FastQC (version 0.11.5)