FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644226
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT47070.2862745145740306No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42750.2600007541542343No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39750.2417550871960424No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA39530.24041707161910833No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37200.2262462702815793No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG37040.22527316804380904No Hit
GTATGGGATGACAGCCTGACTACTTATGTCTTCGGAACTGGGACCGAGGT36230.22034683796509727No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33190.20185789544746283No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCACCATCTCCTG32290.19638419536000526No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG32200.19583682535125949No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA30360.18464614961690182No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC29830.1814227484542879No Hit
CATTAATACTGGTGGTACTACCATATTCTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGT28860.17552331613780586No Hit
ATCCCATACTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG27590.16779931712550464No Hit
GTATTTATACCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAG26900.1636028137251205No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC26680.16226479814818645No Hit
GCCTTGGGCTGACCTCGGACGGAGACCTCGGTCCCAGTTCCGAAGACATAAGTAGTCAGGCTGTCATCCCATACT26090.1586764836464087No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25810.1569735547303108No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25300.15387179134741816No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCAC23980.14584369788581375No Hit
GGACAGAGGGTCACCATCTCCTGCTCTGGAGGCAGCTCCAACATTGGGAGTTATTATGTATCCTGGTACCAGCAG23290.14164719448542962No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22800.13866706888225827No Hit
GTATTAATGTGTGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGAC22510.13690332107629974No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22120.13453138437173479No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21860.13295009323535817No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21520.1308822509800964No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC21220.12905768428427722No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG21120.12844949538567083No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTCGGACGGAGACCTCGGTC20990.12765884981748252No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20060.12200269306044302No Hit
GATTATTACTGCGGAGTATGGGATGACAGCCTGACTACTTATGTCTTCGG19790.12036058303420577No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGGTTCCTGACCGATTCTCTGG19690.11975239413559936No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18890.1148868829467482No Hit
GTATGGGATGACAGCCTGACTACTTATGTCTTCGGAACTGGGACCGAGGTCTCCGTCCGAGGTCAGCCCAAGGC18360.11166348178413431No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAG17680.10752779727361081No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG17670.10746697838375018No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG17600.1070412461547257No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17460.10618978169667673No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG16880.10266228608475964No Hit
ACCATATTCTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGTCAAGAATTCACTGTATCTG16700.10156754606726812No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16580.10083771938894046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12750.047.48871
TACGTAT700.001043511741.53277670
GTATCAA33900.039.2335431
GTGGTAT3050.036.8039631
TGGTATC3100.036.191622
TATCAAC38600.034.1743432
ATCAACG40000.033.0551953
TCAACGC39900.033.0498354
CAACGCA40950.032.2873655
AACGCAG41850.031.836666
CTAATGG950.004668899330.60309670
CGCAGAG44400.029.7776438
AGAGTAC45200.029.40099111
CAGAGTA46000.028.8896710
AATGGTA4800.028.77009870
GCAGAGT46350.028.5981889
TAGGTGG13200.028.0665765
CTGAAGG17400.027.98688370
TCAGGTC13000.027.97461512
ATAGGTG13200.027.8073944
Produced by FastQC (version 0.11.5)