FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729502
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16780.23001993140526No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC11050.1514731967835592No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC10990.15065071788699688No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT8610.11802572165669184No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT7710.10568853820825713No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7530.1032211015185702No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGT407.500769E-570.7742570
TAACGTT200.007408993351.6594540
CGTGCGT901.5595651E-647.1828370
CCTAACG1200.041.7803422
TCGATTG450.001134601540.8298469
CTAACGC1250.040.100883
GGTATCA15950.036.475351
GTATCAA36200.035.098281
TACTCGA603.074463E-431.6710266
TAACGCC1600.031.3288124
CGGTGCA604.2695424E-429.94575954
ATAGTGC450.00540132629.7043536
ACGCCAT1850.028.9015356
TATCAAC43350.028.7595232
ATCAACG43550.028.6982943
AACGCAG43850.028.6543716
TCAACGC43600.028.5887374
CAACGCA44300.028.2878655
TCGCAGT755.2537667E-528.25227549
CCGTGCG1155.0101717E-627.95956669
Produced by FastQC (version 0.11.5)