FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1376030
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35650.2590786538084199No Hit
GAGTATTAGTAGGTGGTTGGCCTGGTATCAACAGAAACCAGGGAAAGCCCCTAAACTCCTAATCTATAAGGCAT31730.23059090281461886No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31030.22550380442286866No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25700.18676918381139945No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20640.14999672972246245No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20580.14956069271745528No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20240.14708981635574805No Hit
GAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTA19360.1406946069489764No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19140.1390958045972835No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19000.13807838491893346No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT17680.12848557080877598No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17440.12674142278874734No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17310.12579667594456517No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16980.12339847241702578No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16610.12070957755281497No Hit
CACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACACTATAATGATTATTCCCGGACTTT16460.1196194850402971No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16230.1179480098544363No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15970.11605851616607195No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGGTGGTTGGCCTGGTATCAACAGAAA15760.11453238664854691No Hit
GTATTAGTAGGTGGTTGGCCTGGTATCAACAGAAACCAGGGAAAGCCCCTAAACTCCTAATCTATAAGGCATCTA15550.11300625713102186No Hit
TCATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTC15190.1103900351009789No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15110.10980865242763603No Hit
CTATAATGATTATTCCCGGACTTTCGGCCAAGGGACCAAGGTGGAACTCAGACGAACTGTGGCTGCACCAT14870.10806450440760738No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT14800.10755579456843238No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14640.10639302922174662No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG14590.10602966505090732No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14580.10595699221673946No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14520.10552095521173231No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC14160.10290473318168936No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA14040.10203265917167505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2800.046.1804961
CGTATGC4150.042.41072541
GCCGTCT3900.041.8909546
TCGTATG3950.041.87837640
CGAGACA4750.040.84961323
ATGCGCA4500.040.1661729
ATATCGT603.030691E-740.0439937
ACGAGAC4700.039.1042222
CCGTCTT4200.038.95659647
CGGCTAT700.001589482138.12525670
TCTATCG700.001589482138.12525670
ACATGCG4750.038.01338627
ATCGTAT655.553102E-737.0669739
TTATACA5350.036.8845182
CATGCGC4900.036.8698428
ATCTCGT3550.036.74056237
ATGCCGT4850.036.43869844
CCCACGA5050.036.38344619
TATGCCG5050.035.6421943
TATACAC5000.035.3824123
Produced by FastQC (version 0.11.5)