FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1376030
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41440.30115622479161064No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32840.2386575874072513No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30770.22361431073450433No Hit
GAGTATTAGTAGGTGGTTGGCCTGGTATCAACAGAAACCAGGGAAAGCCCCTAAACTCCTAATCTATAAGGCAT28140.20450135534835723No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24070.17492351184203833No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20040.14563635967239086No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19370.14076727978314427No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19190.13945916876812278No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18620.13531681722055477No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18410.13379068770302974No Hit
GAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTA17850.1297210089896296No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17120.12441589209537583No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17090.12419787359287224No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16720.12150897872866143No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16590.12056423188447926No Hit
TCATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTC15360.11162547328183252No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15180.11031736226681105No Hit
GTATTAGTAGGTGGTTGGCCTGGTATCAACAGAAACCAGGGAAAGCCCCTAAACTCCTAATCTATAAGGCATCTA14710.10690173906092164No Hit
CACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACACTATAATGATTATTCCCGGACTTT14580.10595699221673946No Hit
CTATAATGATTATTCCCGGACTTTCGGCCAAGGGACCAAGGTGGAACTCAGACGAACTGTGGCTGCACCAT14560.10581164654840373No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14520.10552095521173231No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14460.10508491820672514No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC14260.10363146152336795No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT14260.10363146152336795No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13880.10086989382498929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4000.043.3563081
TACGTAT750.001894642636.78583570
CGACCTA400.00278370934.01360724
CTAACGC400.002795149833.9851653
ATCAACG48650.031.2956943
CGATAAT450.00432094931.0930850
TCAACGC49300.031.014184
CAACGCA50100.030.517835
AACGCAG50900.030.2395346
ACGCAGA53750.028.6361337
CGCAGAG54050.028.4771928
GTATCAA56100.027.8828451
GCAGAGT55550.027.5247339
CGCTACG500.0083330527.18220310
GGTATCA30900.027.1819481
AGAGTAC56700.027.1452611
TATTTCG1050.00982836626.27559370
AATCGTC659.3265687E-426.20245428
GGTACGT759.610452E-426.06785468
GCGTAGA20700.025.65678670
Produced by FastQC (version 0.11.5)