FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863558
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27400.3172919479641205No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG25950.30050095071784405No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24850.28776295280687575No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19190.2222201635558932No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18180.21052436547400405No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCATCTATTTAAATTGGTATCAGCACAAA17940.2077451659297928No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17830.20647136613869596No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15040.1741631714372399No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13670.15829857403903386No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13620.15771957413398985No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13620.15771957413398985No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13520.1565615743239018No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTATCATCTCCAGCTTGGTCC13100.15169797512153207No Hit
ATATTACTGTCAACATTGTGATCATCTGTGCACTTTTGGCCAGGGGACCAAGCTGGAGATGATACGAACTGTGG13100.15169797512153207No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13020.15077157527346166No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12950.14996097540640002No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATGATACGAACTGTGGCTGCACCA12720.14729757584319758No Hit
GGCCAAAAGTGCACAGATGATCACAATGTTGACAGTAATATGTTGCAATA12610.14602377605210073No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12440.14405517637495108No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAT12430.14393937639394228No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT12410.14370777643192467No Hit
GATGATCACAATGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGG12370.14324457650788947No Hit
ACATATTACTGTCAACATTGTGATCATCTGTGCACTTTTGGCCAGGGGAC12190.141160176849731No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGTTGATGGTTAAAGTAAAATTTGTCCCAGACCCACTTCCA11990.13884417722955494No Hit
AGTCAGGACATTAGCATCTATTTAAATTGGTATCAGCACAAACCAGGGAA11860.1373387774764405No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC11720.13571757774231724No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGTTGATGGTTAAAGTAAAATTTGTCCCAGACCCACTTCCA11670.13513857783727323No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT11420.13224357831205316No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11400.13201197835003556No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11130.12888537886279786No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10900.12622197929959542No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10720.12413757964143694No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG10710.12402177966042813No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10640.1232111797933665No Hit
GCACAAACCAGGGAAAGCCCCTAATCTCCTGATCTACGATGCATCCGATTTGCTAAGAGGGGCCCCATCAAGGTT10490.12147418007823446No Hit
GTAATACACGGCCGTGTCCTCAGAGCTCAGGCTGCTCAGCTCCATGTAGGCTGTGTCTGTAGATGTGTCCTCGGT10460.12112678013520807No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10380.12020038028713763No Hit
GTGATCATCTGTGCACTTTTGGCCAGGGGACCAAGCTGGAGATGATACGA10220.11834758059099679No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10100.11695798081889114No Hit
GGTTTGTGCTGATACCAATTTAAATAGATGCTAATGTCCTGACTCGCCTGGCAAGTGATGGTGACT10100.11695798081889114No Hit
GTCTCCTACAGATGCAGACAGGGAGGACGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGAGAGCCAGAG9910.11475778117972389No Hit
AACCAGGGAAAGCCCCTAATCTCCTGATCTACGATGCATCCGATTTGCTA9830.11383138133165345No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9830.11383138133165345No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT9550.11058898186340697No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9460.10954678203432774No Hit
GTGCAGCACCTCACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGC9390.10873618216726613No Hit
GTGGAAGTGGGTCTGGGACAAATTTTACTTTAACCATCAACAGCCTGCAGCCTGAAGATATTGCAACATATTA9330.1080413822812133No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9310.10780978231919569No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9160.10607278260406365No Hit
GATTAGGGGCTTTCCCTGGTTTGTGCTGATACCAATTTAAATAGATGCTA8930.1034093830408612No Hit
GTGCAGCCACAGTTCGTATCATCTCCAGCTTGGTCCCCTGGCCAAAAGTG8890.10294618311682596No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCGTCCTCC8810.10201978326875555No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8750.10132498338270274No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8720.10097758343967632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC651.1175871E-842.54727641
TCGTATG702.1902451E-839.42393540
CGAGCCC1300.038.90651715
ACGAGAC1450.037.2308622
CGAGACA1550.034.84098423
ATCTCCG1600.033.7189810
ATACGTA1255.456968E-1232.3702211
TGAGCGA1057.6215656E-1032.33831
GTATCAA25500.032.148961
TACGTAC1307.2759576E-1231.12521412
TCTCCGA1750.030.82878311
TGCACCG1650.030.6642686
CTTATAC2000.030.3629041
CACGAGA1800.029.9897921
GAATACG1351.2732926E-1129.9724279
CCGAAAC803.6389974E-529.81571468
TATACAC1950.029.4096573
AGACACT1850.029.21649625
AATACGT1402.0008883E-1128.90198310
ATACACA2000.028.6727544
Produced by FastQC (version 0.11.5)