FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863558
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG27130.3141653484768829No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26760.3098807491795571No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23720.274677554952881No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19220.22256756349891957No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18030.20878736575887202No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCATCTATTTAAATTGGTATCAGCACAAA16870.19535456796185088No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15450.17891097065860082No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14940.17300517162715184No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14680.16999437212092297No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14520.16814157242478212No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14120.16350957318443No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13510.15644577434289303No Hit
GTAATACACGGCCGTGTCCTCAGAGCTCAGGCTGCTCAGCTCCATGTAGGCTGTGTCTGTAGATGTGTCCTCGGT12620.14613957603310954No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12470.14440257631797748No Hit
ATATTACTGTCAACATTGTGATCATCTGTGCACTTTTGGCCAGGGGACCAAGCTGGAGATGATACGAACTGTGG12430.14393937639394228No Hit
GGCCAAAAGTGCACAGATGATCACAATGTTGACAGTAATATGTTGCAATA12390.14347617646990707No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTATCATCTCCAGCTTGGTCC12160.14081277690670457No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATGATACGAACTGTGGCTGCACCA12140.14058117694468697No Hit
GATGATCACAATGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGG12050.13953897711560775No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11920.1380335773624933No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAT11830.1369913775334141No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11800.13664397759038768No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11680.13525437781828203No Hit
GGTTTGTGCTGATACCAATTTAAATAGATGCTAATGTCCTGACTCGCCTGGCAAGTGATGGTGACT11610.13444377795122042No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT11600.13432797797021162No Hit
AGTCAGGACATTAGCATCTATTTAAATTGGTATCAGCACAAACCAGGGAA11380.13178037838801795No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11380.13178037838801795No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11320.13108557850196512No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGTTGATGGTTAAAGTAAAATTTGTCCCAGACCCACTTCCA11190.12958017874885067No Hit
ACATATTACTGTCAACATTGTGATCATCTGTGCACTTTTGGCCAGGGGAC11010.12749577909069224No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10990.12726417912867463No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGTTGATGGTTAAAGTAAAATTTGTCCCAGACCCACTTCCA10990.12726417912867463No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC10920.12645357926161302No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG10850.12564297939455138No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10410.12054778023016405No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10350.11985298034411122No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT10250.11869498053402319No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT10150.11753698072393516No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10100.11695798081889114No Hit
GTCTCCTACAGATGCAGACAGGGAGGACGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGAGAGCCAGAG10060.11649478089485593No Hit
GTGATCATCTGTGCACTTTTGGCCAGGGGACCAAGCTGGAGATGATACGA9900.11464198119871508No Hit
GCACAAACCAGGGAAAGCCCCTAATCTCCTGATCTACGATGCATCCGATTTGCTAAGAGGGGCCCCATCAAGGTT9830.11383138133165345No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9820.11371558135064466No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9760.11302078146459185No Hit
GTGGAAGTGGGTCTGGGACAAATTTTACTTTAACCATCAACAGCCTGCAGCCTGAAGATATTGCAACATATTA9680.11209438161652141No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9530.11035738190138937No Hit
AACCAGGGAAAGCCCCTAATCTCCTGATCTACGATGCATCCGATTTGCTA9510.11012578193937177No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9500.11000998195836297No Hit
GATTAGGGGCTTTCCCTGGTTTGTGCTGATACCAATTTAAATAGATGCTA9170.10618858258507247No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA8880.10283038313581717No Hit
GTGTATTACTGTGCAAGCGTCTATGATAGTAGTGGTTGGGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC8650.10016698357261469No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAT400.002450788434.90711646
AATGGTA4550.033.19662570
GTATCAA25500.033.0383951
TGCCTAA900.003769587331.96712170
CCGTTAA951.1083102E-831.91627516
TAGCACC553.7573872E-430.607224
AACGCAG28450.029.7086876
CTAGCAC1152.2046152E-929.2832553
ATCAACG28850.029.0650253
TACACCC704.542085E-528.8632495
TCAACGC29200.028.825294
AACTGTA1255.3936196E-428.77040570
CCGCATA500.00633144328.75439858
CAACGCA29300.028.7319035
ACGCAGA29650.028.6198847
TATCAAC29350.028.4584392
GGTAGAC606.240587E-428.0663682
CGCAGAG30000.028.0614918
GATTCAC855.313783E-627.7522531
TCTCGCG2354.627873E-827.54613370
Produced by FastQC (version 0.11.5)