FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050363
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAACAATATGGTAGCTCATCATTCACTTTCGGCCCTGGGA31110.2961833194809794No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24840.23648967071383892No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24120.2296348976496697No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCTACAGTGAAAATCTCCTGCAATGTTTCTGGATTCACTTTCACCGA19010.1809850499303574No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18360.17479671313631573No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16830.16023032037495608No Hit
GTGTATTACTGTGCAGCAGGTGCGACCTACTACCGGAAGACGGCTGACTA15890.15128103331895734No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15620.1487104934198939No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15020.1429981825330862No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14700.13995161672678874No Hit
CATCTATGCTGCATCCAGAAGGGCCACTGGCACCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA14260.13576258874312977No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14080.13404889547708745No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT14020.13347766438840666No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13970.1330016384811727No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13840.13176397112236435No Hit
GTAATACACGGCCGTGTCTTCAGCTCTCAGGCTGCTCAGCTCCATGTAGGCTGTGTCCGTAAATTTGTCCGCGGT13630.12976466231198167No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13430.12786055868304577No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13300.12662289132423743No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12990.12367153069938679No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCACCTACTTAGCCTGGTACC12480.11881606644560024No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12150.115674295457856No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGGTGCCAGTGGCCCTTCT12100.11519826955062203No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11920.11348457628457971No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11730.11167567783709062No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11730.11167567783709062No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11710.11148526747419701No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11460.10910513793802713No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11460.10910513793802713No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11450.10900993275658034No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11260.10720103430909124No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11110.10577295658738932No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG11060.10529693068015533No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG10900.10377364777700662No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10640.10129831305938995No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10630.10120310787794316No Hit
GTGTATTATTGTCAACAATATGGTAGCTCATCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA10620.10110790269649635No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGC200.00733302451.79641
TAGCACG200.00771201151.1400454
ATCGCGT352.3201226E-549.1140137
GTCCGTT305.7153456E-446.93486455
CACCTAG650.00100681941.8335970
GGTATCA10750.041.2291831
TCGTATG856.730261E-1140.56954240
GTATCAA30350.038.7552221
TAAGACA2400.038.48144531
CCCACGA2600.038.03083419
ATAGCAC451.0739195E-437.881513
CGTATGC1100.037.6698241
ACGAGAC2700.036.6362422
TCTCCGA2800.036.52338811
GACCCTA2650.036.05235726
ATCTCCG2850.035.88262610
CGTAATT951.5018013E-435.77873270
CCCTAAG2700.035.41509228
TATCAAC33450.034.7558672
CATGTCG701.0053336E-634.35836436
Produced by FastQC (version 0.11.5)