FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050363
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAACAATATGGTAGCTCATCATTCACTTTCGGCCCTGGGA28670.27295325520796143No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25420.24201157123775302No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25190.23982185206447676No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20090.19126720952661128No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCTACAGTGAAAATCTCCTGCAATGTTTCTGGATTCACTTTCACCGA19000.1808898447489106No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18200.173273430233167No Hit
GTGTATTACTGTGCAGCAGGTGCGACCTACTACCGGAAGACGGCTGACTA16150.15375636803657403No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15890.15128103331895734No Hit
GTAATACACGGCCGTGTCTTCAGCTCTCAGGCTGCTCAGCTCCATGTAGGCTGTGTCCGTAAATTTGTCCGCGGT15590.1484248778755535No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14730.14023723227112914No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14710.14004682190823553No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14630.13928518045666116No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14440.13747628200917208No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT14140.13462012656576822No Hit
CATCTATGCTGCATCCAGAAGGGCCACTGGCACCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA13950.1328112281182791No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12910.12290988924781243No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12360.1176736042682387No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCACCTACTTAGCCTGGTACC11980.11405580737326047No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11610.11053321565972905No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11600.11043801047828226No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11530.1097715742081547No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11430.10881952239368675No Hit
GTGTATTATTGTCAACAATATGGTAGCTCATCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA11180.10643939285751688No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGGTGCCAGTGGCCCTTCT11050.10520172549870854No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10750.10234557005530469No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG10690.10177433896662391No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG10540.10034626124492199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)