FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023088
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT48880.47776926325008207No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCA39000.3811988802527251No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35150.34356770874059706No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC34390.3361392177408004No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG33740.3297859030699216No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC33360.3260716575700233No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG31310.306034280531098No Hit
GCTTATAACCGTGTCTCCTGGTACCAGCAACTCCCAGGCAAAGCCCCCAAACTCCTGCTTTATGAGGTCATTAAG31200.30495910420218003No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC29910.29235021816305146No Hit
GACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAAAAATTGTTG29140.2848239838606259No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC29100.2844330106501103No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC28580.27935035891340726No Hit
ACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAAAAATTGTTGC28420.27778646607134483No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAAAAATTGTTGCTGGCTCCTTGTGAA26930.2632227139796381No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT26010.25423033013777896No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT24230.23683202226983407No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC23870.23331326337519354No Hit
TCTCTGGCCTCCAGGCTGACGATGAGGCCGATTATTACTGCAGTTCACAA23660.23126065401998652No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG22840.22324570320441645No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG22550.22041114742817822No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACCGTGTCTCCTGGTACCAGCAACTCCCAGGCAAAGC22340.21835853807297126No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21950.21454654927044398No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21160.20682482836276056No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACCGTGTCTCCTGGTACCAGCAACT20420.1995918239682217No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC19730.1928475360868273No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19220.18786262765275324No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG19130.1869829379290931No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC19070.1863964781133197No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG17080.1669455608901678No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15860.15502087796944153No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15420.15072017265376977No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15150.14808110348278936No Hit
GTTATAAGCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG15060.14720141375912923No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG14660.14329168165397307No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14630.14299845174608636No Hit
CCCCTGAGGGCCGCTTAATGACCTCATAAAGCAGGAGTTTGGGGGCTTTGCCTGGGAGTTGCTGGTACCAGGAGA14270.13947969285144582No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA13790.13478801432525844No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT13710.1340060679042272No Hit
CTCCCAGGCAAAGCCCCCAAACTCCTGCTTTATGAGGTCATTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCT13520.13214894515427802No Hit
GTTCACAAGGAGCCAGCAACAATTTTTATGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGG13420.131171512127989No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT13390.13087828222010228No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC13280.12980310589118432No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT13090.12794598314123518No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC12960.12667532020705943No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGCCTCCAGG12950.1265775769044305No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12950.1265775769044305No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12690.12403625103607901No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC12590.12305881800978996No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTG12490.12208138498350093No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12460.12178815507561422No Hit
GGCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG12450.12169041177298531No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12370.12090846535195408No Hit
CTCCTGCTTTATGAGGTCATTAAGCGGCCCTCAGGGGTCCCTGATCGCTT12160.1188558559967471No Hit
CCCTGACCGTCTCTGGCCTCCAGGCTGACGATGAGGCCGATTATTACTGC12120.11846488278623149No Hit
CTCCCTGACCGTCTCTGGCCTCCAGGCTGACGATGAGGCCGATTATTACT11980.11709647654942683No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11930.11660776003628232No Hit
GTCATTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGT11820.11553258370736437No Hit
TTACTGCAGTTCACAAGGAGCCAGCAACAATTTTTATGTCTTCGGAAGTG11720.11455515068107534No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT11660.11396869086530191No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG11590.11328448774689957No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT11580.11318674444427068No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA11510.11250254132586836No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC11160.10908152573385671No Hit
GATTATTACTGCAGTTCACAAGGAGCCAGCAACAATTTTTATGTCTTCGG10990.10741988958916536No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10910.10663794316813412No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT10880.1063447132602474No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG10790.10546502353658727No Hit
GTAATAATCGGCCTCATCGTCAGCCTGGAGGCCAGAGACGGTCAGGGAGG10730.10487856372081385No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA10650.10409661729978263No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG10390.10155529143143112No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT350.00400878360.33096770
TCGTATG2650.049.42823840
GTCGTAT709.094947E-1249.14702239
CGTATGC2500.048.3417741
GGTATCA12200.048.2916561
ATGCCGT2550.047.6218244
CGTAGTA3000.047.5896630
TGTCGTA751.8189894E-1145.78186838
GCGTAGT3200.044.57592429
CGCGTAG3200.044.5453428
GACGCGT3200.044.47995426
AGACGCG3200.044.46907425
ACGCGTA3250.043.82781627
TAACGGC551.5584737E-743.58203536
AGGGGGG3450.042.8437370
CGTCTTC2900.042.18926648
CCGTCTT2900.042.0915447
CGAGACG3500.040.6395523
ATCTCCG3500.040.6196910
TTAACGG603.1207855E-739.89335
Produced by FastQC (version 0.11.5)