FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023088
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC41110.40182271710742384No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT40140.3923416167524201No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCA36750.3592066371612217No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG33020.32274838528064054No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG32970.32225966876749607No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC32380.3164928139123907No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32180.31453794785981265No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC31610.3089665796099651No Hit
GCTTATAACCGTGTCTCCTGGTACCAGCAACTCCCAGGCAAAGCCCCCAAACTCCTGCTTTATGAGGTCATTAAG30240.29557574714980533No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28480.2783729258871182No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC27530.26908731213737236No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27340.26723018938742316No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAAAAATTGTTGCTGGCTCCTTGTGAA26210.256185196190357No Hit
ACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAAAAATTGTTGC25620.2504183413352517No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25400.2482679886774158No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT25340.2476815288616424No Hit
GACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAAAAATTGTTG24710.24152370079602148No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC24120.23575684594091614No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23310.22783963842797494No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACCGTGTCTCCTGGTACCAGCAACTCCCAGGCAAAGC22720.22207278357286958No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22340.21835853807297126No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22210.21708787513879552No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC21790.21298265642838154No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20180.197245984705128No Hit
TCTCTGGCCTCCAGGCTGACGATGAGGCCGATTATTACTGCAGTTCACAA20140.19685501149461238No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG18990.18561453169228845No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18340.1792612170214097No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACCGTGTCTCCTGGTACCAGCAACT18030.17623117463991367No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG17800.1739830786794489No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16510.16137419264032027No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16060.1569757440220196No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15010.1467126972459847No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14750.1441713713776332No Hit
GTTATAAGCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG14340.14016389596984816No Hit
CCCCTGAGGGCCGCTTAATGACCTCATAAAGCAGGAGTTTGGGGGCTTTGCCTGGGAGTTGCTGGTACCAGGAGA14220.1389909763383013No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14210.13889323303567241No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13990.1367428803778365No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC13750.13439704111474282No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT13620.13312637818056705No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG13430.1312692554306179No Hit
GTTCACAAGGAGCCAGCAACAATTTTTATGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGG13310.13009633579907104No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGCCTCCAGG13070.12775049653597736No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12810.12520917066762585No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC12700.1241339943387079No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12670.1238407644308212No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC12460.12178815507561422No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC12300.12022426223355176No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT12130.11856262608886038No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC12050.11778067966782917No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTG12010.11738970645731354No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12010.11738970645731354No Hit
GGCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG11960.11690098994416903No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT11950.11680324664154011No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11940.11670550333891122No Hit
CTCCCAGGCAAAGCCCCCAAACTCCTGCTTTATGAGGTCATTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCT11850.11582581361525109No Hit
CCCTGACCGTCTCTGGCCTCCAGGCTGACGATGAGGCCGATTATTACTGC11820.11553258370736437No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11780.11514161049684876No Hit
CTCCTGCTTTATGAGGTCATTAAGCGGCCCTCAGGGGTCCCTGATCGCTT11770.11504386719421984No Hit
GTCATTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGT11720.11455515068107534No Hit
CTCCCTGACCGTCTCTGGCCTCCAGGCTGACGATGAGGCCGATTATTACT11640.1137732042600441No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG11600.11338223104952849No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11540.11279577123375506No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC11380.11123187839169262No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA11350.1109386484838059No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11080.1082995793128255No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT10860.10614922665498959No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA10800.10556276683921617No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG10780.10536728023395837No Hit
TTACTGCAGTTCACAAGGAGCCAGCAACAATTTTTATGTCTTCGGAAGTG10760.10517179362870056No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10720.10478082041818496No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA10490.10253272445772015No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG10480.10243498115509125No Hit
GACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAAAAATTGTTGCTGGCTCCTTGTGAACTGCAGTAAT10470.10233723785246236No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT10430.10194626464194673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)