FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769151
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC67740.3828955244634291No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAGCCGC58450.33038446124723103No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG55160.3117879706141533No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT51160.28917825555873977No Hit
GATTATTACTGCTGCTCATATGCAGGCAGTTACACTTTCTATGCGGCTTTCGGCGGAGGGACCAAGCTGACCGTC45520.25729855733060664No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC45510.25724203304296805No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG44010.24876338989718796No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAGCCGCATAGAAAGTG41530.23474536656283157No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG39400.22270569329582382No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC37870.2140574772871281No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT37030.2093094371254913No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG36110.20410920266274615No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC35750.20207432830775893No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCAGTTACACTTTCTATGCGG35200.19896549248763956No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGGGATGTT34570.19540446236641193No Hit
CTCCTGCACTGGAACCAGCAGGGATGTTGGTGGTTATAATTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC33640.19014770361602826No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT32560.18404308055106658No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG31600.17861674893776733No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31460.17782540891082785No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG30640.17319041732446805No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC30130.17030767865490284No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC30010.16962938720324042No Hit
GTGTCCGGATCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG29220.16516396847979622No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28910.16341171556300169No Hit
GTGTAACTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGC28370.16035940403052085No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG27210.1538025866644509No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG27140.15340691665098116No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGATCTCCTGGACAGTCAGTCAC27110.15323734378806558No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGGGATGTTGGTGGTTATAATTATGTCTCCTGGTACCAACAACA26920.15216338232293342No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26830.15165466373418662No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25780.14571961353214055No Hit
GTAATAGACGGCCGTGTCCTCAGGTCTCAGGCTGCTCACCTCCAAGTAGACTGTATTCGTCGACGTGTCCCTGGT25310.14306297201312945No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAGCCGCATAGAAAGTGT25310.14306297201312945No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGATCTCCTGGACAGTCAGTCACCATCTCCTG25230.1426107777120212No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24400.1379192618380229No Hit
CCCCTGAGGGCCGCTTATTGACATCATAAATCATGACTCTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA24330.13752359182455312No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23070.13040153158209786No Hit
GTCTATTACTGTGCGAGAGGGGGATCTAGTGACAACGTTAAGCCTTCTCACTTTGGCCACTGGGGCCAGGGAACC22990.1299493372809896No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC22750.12859275437766476No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22420.12672745288559314No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21460.12130112127229388No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC21000.11870100404092132No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGATCTCCTGGACAGTCAGTCAC20980.11858795546564424No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20640.1166661296859341No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC20440.11553564393316341No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG20430.11547911964552489No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20200.1141790610298386No Hit
CAGTTACACTTTCTATGCGGCTTTCGGCGGAGGGACCAAGCTGACCGTCC20160.11395296387928448No Hit
GGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCAGTTACACTTTCT19860.11225723525012846No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19710.11140937093555045No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19550.11050498233333389No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19480.11010931231986416No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19070.10779181652668428No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG18990.107339622225576No Hit
GACTCAGCCTCGCTCAGTGTCCGGATCTCCTGGACAGTCAGTCACCATCT18840.10649175791099799No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT18790.10620913647280532No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18660.10547432073350438No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18630.10530474787058877No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18260.10321334922796302No Hit
GCCTCGCTCAGTGTCCGGATCTCCTGGACAGTCAGTCACCATCTCCTGCA18170.10270463063921621No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC18110.10236548491338501No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)