FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358202
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51350.3780733646394277No Hit
GTATTACTGTCAGCAGTGTGATAGCTCACCTCGGACGTTCGGCCAAGGGA30790.2266967652823365No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30140.22191102648943234No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT27020.19893948028349243No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24140.17773497609339406No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23520.1731701175524701No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23150.17044592777804773No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23010.16941515326880685No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAAACCCACTGCCA22340.16448216097458257No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19330.14232050902590337No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT18330.13495783395989697No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18220.13414793970263628No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17680.13017209516699282No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17240.12693251813795No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT16770.12347206085692702No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16750.1233248073556069No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA16690.12288304685164651No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16290.11993797682524397No Hit
GTGTATTACTGTCAGCAGTGTGATAGCTCACCTCGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAA16250.1196434698226037No Hit
CTCCTGCACGGCCAGTCAGAGTGTTAGCAGCAGCTTCTTAGCCTGGTACC16120.11868632206402288No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16010.11787642780676218No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15860.11677202654686122No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15360.11309068901385803No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14560.10720054896105292No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC14220.10469723943861076No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14190.10447635918663055No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG13910.10241481016814878No Hit
GTGTGATAGCTCACCTCGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT13880.10219392991616857No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC13620.10027963439900693No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13600.10013238089768678No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG13590.10005875414702674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19600.041.5627561
CCTAACG504.4452954E-640.532612
GTATCAA54700.037.4479981
ATGGCAA7150.034.7912470
TTCGCAC400.002870658233.8008317
CTAACGC601.5549418E-533.7684673
TATCAAC60600.033.4427452
ATCAACG62100.032.409033
TCAACGC62400.032.084964
CAACGCA62900.031.7809165
AACGCAG63100.031.6801836
TACGTAT2051.8158971E-830.33626670
CGCAGAG66000.030.2881778
AGAGTAC67250.029.57456611
ACGCAGA68000.029.347697
GCAGAGT67700.029.3281049
AATGGTA9150.028.69696670
TAGTCGC754.9919778E-528.46772455
CACTGAT5400.028.15155870
CAGAGTA71250.027.91423810
Produced by FastQC (version 0.11.5)