EGAF00004692648 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00004692648
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	634781
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTATTACTGTCAGCAGTATGGTGTCTCACCCTCTTTCGGCGGAGGGACCACGGTGGAGATCAAACGAACTGTGG	2324	0.36611051685541945	No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT	2167	0.3413775774637237	No Hit
CAGTAATACACTGCAACATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA	1681	0.2648157396015319	No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC	1407	0.22165124665041958	No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC	1393	0.21944576160912188	No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC	1284	0.20227448521616115	No Hit
GTGTATTACTGTCAGCAGTATGGTGTCTCACCCTCTTTCGGCGGAGGGAC	1283	0.20211695057035417	No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA	1264	0.19912379230002156	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1202	0.18935664425998888	No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT	1148	0.180849773386412	No Hit
GTAATACACTGCAACATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA	1130	0.1780141497618864	No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATG	1099	0.17313057574187002	No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG	1096	0.17265797180444908	No Hit
CTCCTGCAGGGCCAGTCAGCGTGTTAGCAACAGTTACTTAGCCTGGTACC	1079	0.16997988282573046	No Hit
GTATGGTGTCTCACCCTCTTTCGGCGGAGGGACCACGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT	1017	0.16021273478569775	No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT	989	0.15580176470310234	No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA	964	0.15186339855792785	No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC	926	0.14587708201726265	No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT	915	0.1441442009133859	No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT	900	0.1417811812262812	No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGCGTGTTAGCAA	896	0.14115104264305328	No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA	892	0.14052090405982537	No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC	868	0.13674007256045786	No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT	860	0.13547979539400204	No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG	844	0.13295924106109036	No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT	804	0.12665785522881118	No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGCTTTGTGCTGGTACCAGGCTAAGTAACTGTTGCTAACACGCTGA	774	0.12193181585460182	No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC	774	0.12193181585460182	No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG	767	0.12082907333395296	No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGC	760	0.11972633081330411	No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC	742	0.11689070718877849	No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG	740	0.11657563789716453	No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT	727	0.1145276875016738	No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT	713	0.1123222024603761	No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCCCTCCGCCGAAAGAG	708	0.1115345292313412	No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA	705	0.11106192529392028	No Hit
GTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAG	689	0.1085413709610086	No Hit
ATGTATTACTGTGCGAGACAAGGGGGTTTGGGATGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTC	681	0.10728109379455276	No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCC	677	0.10665095521132485	No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG	676	0.10649342056551786	No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA	675	0.1063358859197109	No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATGTTGCAGTGTATTACTGTCAGCAGTATGGTGTCTCA	640	0.10082217331646662	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACCGT	20	0.0068868464	52.62227	50
GTCTAGC	20	0.007936534	50.767796	1
GGTACGG	20	0.00795882	50.7318	3
GGTATCA	835	0.0	46.207813	1
CCTAACG	60	3.3920332E-7	39.467396	2
ATCGTTG	80	1.6552804E-9	38.76969	40
TAATCGT	80	1.7316779E-9	38.5931	38
AATCGTT	85	3.0522642E-9	36.421875	39
TTAATCG	85	3.1686795E-9	36.282326	37
TCACGTA	75	4.7208232E-8	36.13003	25
CGTAGGA	85	3.4851837E-9	35.929573	28
GTATCAA	1990	0.0	35.54596	1
CGTTGAA	80	6.9303496E-8	34.58689	42
ACGTAGG	80	8.275856E-8	33.895973	27
CACGTAG	80	8.304596E-8	33.882595	26
ATTGCCG	40	0.0028456147	33.858543	20
CCGGTCT	90	6.2718755E-9	33.831863	9
CTAACGC	60	1.5371352E-5	33.8212	3
ACGAACC	95	7.1449904E-9	33.38243	52
AATTTCG	65	2.1372582E-5	32.230175	47

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content