FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325415
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68370.5158384355088783No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC61310.4625721000592268No Hit
CCATTAACAGTCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAGCTGAGTTACAGTACTCCTCCGGAGAACA47290.35679390983201487No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG45570.34381684227204307No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43280.32653923488115044No Hit
CTCCTAATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCTTCAAGGTT41820.31552381706861626No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38910.293568429510757No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37910.28602362278984317No Hit
GATTAGGAGTTTAGGGGCTTTCCCTGGATGTTGCTGATACCAATTTAAAT36680.2767435105231192No Hit
GTCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAGCTGAGTTACAGT35870.27063221707917895No Hit
CTAGGAGACAGCGTCACCATCTCTTGCCGGGCAAGTCAGAACATTAGGTCTCATTTAAATTGGTATCAGCAACAT35180.2654263004417484No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTACTCTGG33970.2562970843094427No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33630.253731850024332No Hit
GAGTTACAGTACTCCTCCGGAGAACACTTTTGGTCAGGGGACCAAGTTGG33100.24973310246224767No Hit
GTACTGTAACTCAGCTGACAATAGTAAGTTGCAAAATCTTCAGGCTGTAGACTGTTAATGGTGAGAGTGAAAT32360.24414994548877145No Hit
GTCTCCATCCTCCCTGTCTGCGTCTCTAGGAGACAGCGTCACCATCTCTTGCCGGGCAAGTCAGAACATTAGGT32340.24399904935435315No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31700.2391703730529683No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31570.2381895481792495No Hit
GTTAATGGTGAGAGTGAAATCTGTGCCAGAGCCACTGGCACTGAACCTTG31240.23569976196134795No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31220.2355488658269297No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31020.2340399044827469No Hit
CTGTTAATGGTGAGAGTGAAATCTGTGCCAGAGCCACTGGCACTGAACCT29860.2252879286864869No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29570.22309993473742187No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGGTGTGACATCCAGATGACCCAGTC28420.21442340700837098No Hit
GATTTCACTCTCACCATTAACAGTCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAGCTGAGTTACAGTACT27960.21095279591675062No Hit
GTTCAGTGCCAGTGGCTCTGGCACAGATTTCACTCTCACCATTAACAGTCTACAGCCTGAAGATTTTGCAACTTA27850.21012286717745007No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27750.20936838650535872No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCT27030.20393612566630076No Hit
CTTCAAGGTTCAGTGCCAGTGGCTCTGGCACAGATTTCACTCTCACCATTAACAGTCTACAGCCTGAAGATTTTG26850.20257806045653626No Hit
CACCATTAACAGTCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAGCTGAGTTACAGTACTCCTCCGGAGAA26110.19699490348306No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25960.19586318247492296No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTACTCTGGCTCCGAGGTGCCAGGTGTGACAT25840.19495780566841328No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25610.1932225001226031No Hit
CTCTAGGAGACAGCGTCACCATCTCTTGCCGGGCAAGTCAGAACATTAGG25330.19110995424074723No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAACTTGGTCCCCTGACCAAAAGTG25260.19058181777028327No Hit
CTGTAACTCAGCTGACAATAGTAAGTTGCAAAATCTTCAGGCTGTAGACTGTTAATGGTGAGAGTGAAATCTGTG25190.1900536812998193No Hit
GAGTGAAATCTGTGCCAGAGCCACTGGCACTGAACCTTGAAGGGACCCCA24740.18665851827540808No Hit
AATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCTTCAAGGTTCAGTGCCAGTGGCTCTGGCACAGATTTCA24170.1823579784444872No Hit
GACCAAAAGTGTTCTCCGGAGGAGTACTGTAACTCAGCTGACAATAGTAAGTTGCAAAATCTTCAGGCTGTAGA23530.17752930214310234No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23410.17662392533659269No Hit
GTTACAGTACTCCTCCGGAGAACACTTTTGGTCAGGGGACCAAGTTGGAGATCAGACGAACTGTGGCTGCACCAT23140.17458682752194596No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22630.1707389760942799No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22190.1674192611370778No Hit
ACCTTGAAGGGACCCCACTTTGCAAATTGGATGCAGCATAGATTAGGAGTTTAGGGGCTTTCCCTGGATGTTG22140.16704202080103214No Hit
AGTCAGAACATTAGGTCTCATTTAAATTGGTATCAGCAACATCCAGGGAAAGCCCCTAAACTCCTAATCTATG22030.1662120920617316No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCC22000.1659857478601042No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAACTTGGTCC21870.1650049229863854No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21820.1646276826503397No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21490.16213789643243814No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21460.16191155223081072No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21400.1614588638275559No Hit
GTACTCCTCCGGAGAACACTTTTGGTCAGGGGACCAAGTTGGAGATCAGA21260.16040259088662795No Hit
GCTCCTGGGACTCCTGCTACTCTGGCTCCGAGGTGCCAGGTGTGACATCCAGATGACCCAGTCTCCATCCTCC21160.1596481102145366No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21100.15919542181128177No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21090.1591199737440726No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20290.15308412836734156No Hit
CTTTTGGTCAGGGGACCAAGTTGGAGATCAGACGAACTGTGGCTGCACCA19860.1498398614773486No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19620.1480291078643293No Hit
GTGCCAGTGGCTCTGGCACAGATTTCACTCTCACCATTAACAGTCTACAG19030.14357767189899012No Hit
GAGGAGTACTGTAACTCAGCTGACAATAGTAAGTTGCAAAATCTTCAGGC18150.13693824198458596No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACT17480.13188322148157372No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17470.13180777341436456No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17430.131505981145528No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17410.13135508501110973No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17390.13120418887669147No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTACTCTGGCTCC17390.13120418887669147No Hit
GTTTAGGGGCTTTCCCTGGATGTTGCTGATACCAATTTAAATGAGACCTA17240.1300724678685544No Hit
GCCACAGTTCGTCTGATCTCCAACTTGGTCCCCTGACCAAAAGTGTTCTCCGGAGGAGTACTGTAACTCAGCTGA17190.12969522753250867No Hit
GCATAGATTAGGAGTTTAGGGGCTTTCCCTGGATGTTGCTGATACCAATT17140.129317987196463No Hit
CATTTAAATTGGTATCAGCAACATCCAGGGAAAGCCCCTAAACTCCTAAT17000.12826171425553506No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT16780.12660185677693403No Hit
CCATCCTCCCTGTCTGCGTCTCTAGGAGACAGCGTCACCATCTCTTGCCG16520.12464020702949642No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16370.12350848602135935No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTC16260.12267855728205883No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC16130.12169773240834003No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15930.12018877106415728No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT15720.11860436165276537No Hit
CCCCTAAACTCCTAATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCT15550.11732174451021002No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15410.11626547156928208No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15370.11596367930044552No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15360.1158882312332364No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15340.11573733509881809No Hit
GGCTGTAGACTGTTAATGGTGAGAGTGAAATCTGTGCCAGAGCCACTGGC15230.1149074063595176No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14880.11226672400719774No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14850.11204037980557034No Hit
CTATAATAGATGTGGCCAATCCATTCTGGGCCCTTCCCTGGCGGCTGCCG14850.11204037980557034No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14810.11173858753673377No Hit
CTATTATAGTGGTCGCACCTATTACAACCCGTCCCTCGAGAGTCGAGTCACCATCTCTGTGGACGGGTCCAAGAA14630.1103805223269693No Hit
GTCTCCTAGAGACGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT14500.10939969745325048No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14490.10932424938604136No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14410.10872066484836825No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14390.10856976871394997No Hit
CATCCAGGGAAAGCCCCTAAACTCCTAATCTATGCTGCATCCAATTTGCA14320.10804163224348601No Hit
GCACTGAACCTTGAAGGGACCCCACTTTGCAAATTGGATGCAGCATAGAT14210.10721170350418549No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14090.10630632669767581No Hit
GTGGGGTCCCTTCAAGGTTCAGTGCCAGTGGCTCTGGCACAGATTTCACT14090.10630632669767581No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14010.10570274216000271No Hit
CAATAGTAAGTTGCAAAATCTTCAGGCTGTAGACTGTTAATGGTGAGAGTGAAATCTGTGCCAGAGCCACTGGCA13970.10540094989116616No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13960.10532550182395703No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13630.10283571560605546No Hit
CATTAGGTCTCATTTAAATTGGTATCAGCAACATCCAGGGAAAGCCCCTA13580.10245847527000976No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13550.10223213106838236No Hit
GTGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCGTCTCTAG13500.10185489073233665No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGT13370.10087406585861786No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC13350.10072316972419959No Hit
ATCCAGGGAAAGCCCCTAAACTCCTAATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCTTCAAGGTTCAGTG13350.10072316972419959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)