FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325415
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68750.5187054620628256No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50760.38297438915358584No Hit
CTCCTAATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCTTCAAGGTT44900.3387618217690308No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG43150.32555841000743163No Hit
CCATTAACAGTCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAGCTGAGTTACAGTACTCCTCCGGAGAACA42300.31914532429465486No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41380.31220410211141414No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40950.30895983522142123No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38900.2934929814435479No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37840.2854954863193792No Hit
GATTAGGAGTTTAGGGGCTTTCCCTGGATGTTGCTGATACCAATTTAAAT37640.28398652497519644No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35590.2685196711973231No Hit
GAGTTACAGTACTCCTCCGGAGAACACTTTTGGTCAGGGGACCAAGTTGG34730.2620311374173372No Hit
GTCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAGCTGAGTTACAGT34250.2584096301912986No Hit
GTCTCCATCCTCCCTGTCTGCGTCTCTAGGAGACAGCGTCACCATCTCTTGCCGGGCAAGTCAGAACATTAGGT33400.2519965444785218No Hit
GTACTGTAACTCAGCTGACAATAGTAAGTTGCAAAATCTTCAGGCTGTAGACTGTTAATGGTGAGAGTGAAAT32700.24671517977388216No Hit
CTAGGAGACAGCGTCACCATCTCTTGCCGGGCAAGTCAGAACATTAGGTCTCATTTAAATTGGTATCAGCAACAT32050.24181105540528816No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTACTCTGG31220.2355488658269297No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30720.2317764624664728No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30590.23079563759275398No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29400.22181731759486653No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29150.21993111591463807No Hit
CTGTTAATGGTGAGAGTGAAATCTGTGCCAGAGCCACTGGCACTGAACCT29140.21985566784742894No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGGTGTGACATCCAGATGACCCAGTC28610.2158569202853446No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27960.21095279591675062No Hit
GTTAATGGTGAGAGTGAAATCTGTGCCAGAGCCACTGGCACTGAACCTTG27900.21050010751349577No Hit
GTTCAGTGCCAGTGGCTCTGGCACAGATTTCACTCTCACCATTAACAGTCTACAGCCTGAAGATTTTGCAACTTA27490.20740673675792112No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAACTTGGTCCCCTGACCAAAAGTG27290.20589777541373833No Hit
GATTTCACTCTCACCATTAACAGTCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAGCTGAGTTACAGTACT27210.2052941908760652No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCT27150.2048415024728104No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25980.19601407860934122No Hit
CTTCAAGGTTCAGTGCCAGTGGCTCTGGCACAGATTTCACTCTCACCATTAACAGTCTACAGCCTGAAGATTTTG25440.19193988298004777No Hit
CACCATTAACAGTCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAGCTGAGTTACAGTACTCCTCCGGAGAA24680.18620582987215326No Hit
CTGTAACTCAGCTGACAATAGTAAGTTGCAAAATCTTCAGGCTGTAGACTGTTAATGGTGAGAGTGAAATCTGTG24520.18499866079680702No Hit
AATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCTTCAAGGTTCAGTGCCAGTGGCTCTGGCACAGATTTCA24490.18477231659517962No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24280.18318790718378772No Hit
GACCAAAAGTGTTCTCCGGAGGAGTACTGTAACTCAGCTGACAATAGTAAGTTGCAAAATCTTCAGGCTGTAGA24220.1827352187805329No Hit
GAGTGAAATCTGTGCCAGAGCCACTGGCACTGAACCTTGAAGGGACCCCA24130.18205618617565064No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23970.18084901710030443No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23800.17956639995774906No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23780.1794155038233308No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTACTCTGGCTCCGAGGTGCCAGGTGTGACAT23390.17647302920217442No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23230.17526586012682818No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22820.17217248937125354No Hit
CTCTAGGAGACAGCGTCACCATCTCTTGCCGGGCAAGTCAGAACATTAGG22760.1717198009679987No Hit
ACCTTGAAGGGACCCCACTTTGCAAATTGGATGCAGCATAGATTAGGAGTTTAGGGGCTTTCCCTGGATGTTG22370.1687773263468423No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAACTTGGTCC22220.16764560533870523No Hit
AGTCAGAACATTAGGTCTCATTTAAATTGGTATCAGCAACATCCAGGGAAAGCCCCTAAACTCCTAATCTATG20930.1579128046687264No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20840.15723377206384415No Hit
GTTACAGTACTCCTCCGGAGAACACTTTTGGTCAGGGGACCAAGTTGGAGATCAGACGAACTGTGGCTGCACCAT20720.1563283952573345No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20550.15504577811477915No Hit
CTTTTGGTCAGGGGACCAAGTTGGAGATCAGACGAACTGTGGCTGCACCA20470.15444219357710604No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCC20340.15346136870338725No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20300.15315957643455066No Hit
GCTCCTGGGACTCCTGCTACTCTGGCTCCGAGGTGCCAGGTGTGACATCCAGATGACCCAGTCTCCATCCTCC20200.1524050957624593No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20160.15210330349362275No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19800.14938717307409377No Hit
GTACTCCTCCGGAGAACACTTTTGGTCAGGGGACCAAGTTGGAGATCAGA19220.14501118517596376No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19130.1443321525710815No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18900.14259684702527134No Hit
GTGGGGTCCCTTCAAGGTTCAGTGCCAGTGGCTCTGGCACAGATTTCACT18860.14229505475643478No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18580.14018250887457892No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18560.14003161274016063No Hit
GAGGAGTACTGTAACTCAGCTGACAATAGTAAGTTGCAAAATCTTCAGGC18280.13791906685830477No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17990.13573107290923975No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA17960.13550472870761235No Hit
GTTTAGGGGCTTTCCCTGGATGTTGCTGATACCAATTTAAATGAGACCTA16870.12728088938181625No Hit
GTGCCAGTGGCTCTGGCACAGATTTCACTCTCACCATTAACAGTCTACAG16820.1269036490457706No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16590.12516834349996037No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16550.12486655123112383No Hit
GCCACAGTTCGTCTGATCTCCAACTTGGTCCCCTGACCAAAAGTGTTCTCCGGAGGAGTACTGTAACTCAGCTGA16550.12486655123112383No Hit
GGCTGTAGACTGTTAATGGTGAGAGTGAAATCTGTGCCAGAGCCACTGGC16540.1247911031639147No Hit
GCATAGATTAGGAGTTTAGGGGCTTTCCCTGGATGTTGCTGATACCAATT16460.1241875186262416No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTACTCTGGCTCC16010.12079235560183037No Hit
CTATAATAGATGTGGCCAATCCATTCTGGGCCCTTCCCTGGCGGCTGCCG15880.11981153072811157No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15430.11641636770370034No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15430.11641636770370034No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15410.11626547156928208No Hit
CCATCCTCCCTGTCTGCGTCTCTAGGAGACAGCGTCACCATCTCTTGCCG15370.11596367930044552No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15170.11445471795626275No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15030.11339844501533482No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTC14990.11309665274649827No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC14920.11256851627603429No Hit
GTGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCGTCTCTAG14900.11241762014161602No Hit
CATTTAAATTGGTATCAGCAACATCCAGGGAAAGCCCCTAAACTCCTAAT14880.11226672400719774No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14800.11166313946952464No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14780.11151224333510637No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14750.11128589913347894No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14750.11128589913347894No Hit
CCCCTAAACTCCTAATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCT14730.11113500299906066No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT14670.11068231459580584No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14510.10947514552045964No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACT14430.10887156098278651No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14350.10826797644511342No Hit
GTCTCCTAGAGACGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT14180.10698535930255806No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14120.10653267089930324No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14050.10600453442883927No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14030.10585363829442099No Hit
CTATTATAGTGGTCGCACCTATTACAACCCGTCCCTCGAGAGTCGAGTCACCATCTCTGTGGACGGGTCCAAGAA13660.10306205980768288No Hit
CAATAGTAAGTTGCAAAATCTTCAGGCTGTAGACTGTTAATGGTGAGAGTGAAATCTGTGCCAGAGCCACTGGCA13660.10306205980768288No Hit
GCACTGAACCTTGAAGGGACCCCACTTTGCAAATTGGATGCAGCATAGAT13500.10185489073233665No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13460.10155309846350011No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTCG150.002551016467.62710616
CGATTGC306.6506873E-660.65313363
TACGTAT651.3491526E-553.82287270
GTACGTA556.708183E-541.0310469
TGGTACG652.6398266E-740.75413567
GTGGTAT3100.040.3657341
CCTAACG451.12846385E-437.5649452
GTATCAA35400.036.9726451
AATGGTA7100.035.4776170
CGAAACC1206.2464096E-933.8506169
TATCAAC39150.033.160782
ATCAACG39500.032.948813
GGTACGT851.4341022E-632.8320568
TCAACGC40050.032.6626784
CAACGCA40600.032.2189875
GGTACTA450.003758838431.99094660
AACGCAG41300.031.9184286
CGGGTCA751.953038E-631.55931717
CGCAGAG42650.030.6715168
TCGCACC5100.030.48354313
Produced by FastQC (version 0.11.5)