FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232988
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51450.4172790002822412No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCC47590.3859729372873053No Hit
GTGCAGTAATAATCAGCCTCATCCTCAGACTGGAGTCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG46390.3762404824702268No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA43500.35280148711909604No Hit
TTACTGCACAGCGTGGGATGACAGCCTGGATGGTCTCGTCTTCGGAACTG43390.35190934542753055No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT43240.3506927885753957No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC41610.3374728707821974No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG39330.3189812066297482No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG37850.30697784568868475No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC36400.29521779611804816No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC35740.28986494596865503No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC35510.2879995587953816No Hit
GATTATTACTGCACAGCGTGGGATGACAGCCTGGATGGTCTCGTCTTCGG33370.27064334770492493No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT32720.2653716013456741No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC32200.2611542042582734No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31110.25231389113276037No Hit
ACCTAGGACGGTGACCTCGGTCCCAGTTCCGAAGACGAGACCATCCAGGC27850.22587405554636378No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTCGGTCCCAGTTCCGAAGACGAGACCATCCAGGCTGTCATCCCACG27790.22538743280550985No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27130.2200345826561167No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGTCCACTGATGGCCAGGGAGG25760.20892336340661874No Hit
GACCTAGGACGGTGACCTCGGTCCCAGTTCCGAAGACGAGACCATCCAGG24800.20113739955295593No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG24360.19756883278669377No Hit
GATTAGTAGTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG23570.19116163336545042No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23400.18978286893303098No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCAC22970.1862954059569112No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22670.18386229225264156No Hit
CTACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG22320.1810236595976603No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22130.17948268758495622No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT22100.17923937621452926No Hit
CATCTATACTACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC21660.17567080944826713No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG21630.17542749807784017No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTCGGTC21620.17534639428769785No Hit
GCGTGGGATGACAGCCTGGATGGTCTCGTCTTCGGAACTGGGACCGAGGT21410.1736432146947091No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21000.17031795929887394No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG20440.165776147050904No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19210.1558003808633985No Hit
CCCCTGAGGGCCGCTGATTAGTAGTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTA18830.15271843683799033No Hit
GAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAA18240.14793331321959338No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA17740.14387812371247732No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT17390.1410394910574961No Hit
GTAATAAACGGCCGTGTCTGCGGCGGTCACAGAGGTCACCTTCAGGGAGA17170.13925520767436503No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16980.13771423566166094No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16670.135200018167249No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG16490.13374014994468722No Hit
GTAGTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCA16390.132929112043264No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCC16100.1305771021291367No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGT16090.1304959983389944No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15800.12814398842486707No Hit
GTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTACAGTATTACTTCCGATGTTGGAG15170.12303444964590084No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG14460.11727608054579607No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14430.1170327691753691No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC14400.11678945780494213No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT14210.11524848579223804No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA14140.1146807592612418No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14130.11459965547109949No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT13900.1127342682978261No Hit
CTATACTACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC13600.11030115459355647No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT13550.10989563564284487No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13510.10957122048227558No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGTCTGAGGATG13280.1077058333090022No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13180.106894795407579No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA13150.10665148403715202No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13020.10559713476530186No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC13020.10559713476530186No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12950.1050294082343056No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12930.10486720065402096No Hit
GCGTGGGATGACAGCCTGGATGGTCTCGTCTTCGGAACTGGGACCGAGGTCACCGTCCTAGGTCAGCCCAAGGC12890.10454278549345168No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12870.10438057791316703No Hit
GTTTATTACTGTGCGAGAGAAAACTACAACTACGGGCGATTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT12640.10251519073989365No Hit
GTGGACTCCAGTCTGAGGATGAGGCTGATTATTACTGCACAGCGTGGGAT12490.10129863388775884No Hit
GTCTCTGCTGGCTCCATCAGCAGTGATAATTACTACTGGAGCTGGATCCG12480.10121753009761651No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC12370.10032538840605099No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)