FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106743
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGA42380.3829253946038059No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32130.2903113008169015No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27350.24712150878749625No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24280.21938245825815025No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22750.20555811060020257No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22330.20176319163527578No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20240.18287895202409232No Hit
GTATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT19390.17519875888078804No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA18070.16327187070530377No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18060.1631815154918531No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17770.1605612143017846No Hit
GTATAGGAGACTACGCACAGAAATTCCAGGGCAGAATTACGATTACCGCG17660.1595673069538276No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17370.15694700576375906No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17370.15694700576375906No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT17190.1553206119216476No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16940.1530617315853816No Hit
GTGTATTACTGTGCGAGAGATGGGTCCAAATCCCCGGTGTTTTGGGGCCA16410.14827290527249778No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA16370.14791148441869523No Hit
CATCTATGGTGCCTCCAGCAGGGCCACTGGCATCCCGGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA16230.14664651143038626No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16070.14520082801517606No Hit
CTCCTGCAGGGCCAGTCAGAATGTTGGCAGCGACTATTTAGCCTGGTACC15320.13842418700637818No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14500.13101505950342582No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14330.12947902087476496No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14250.12875617916715984No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14190.12821404788645602No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA14150.12785262703265346No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13990.12640694361744326No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13760.12432877370807857No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13730.12405770806772666No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13300.1201724338893492No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13170.1189978161144909No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13040.1178231983396326No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12680.1145704106554096No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12610.11393792416125514No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12480.11276330638639684No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12380.11185975425189046No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12250.11068513647703217No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG12190.11014300519632833No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC12170.10996229476942705No Hit
GTCTTTGTCCCCCGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA12140.10969122912907513No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12140.10969122912907513No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12140.10969122912907513No Hit
GTAATACACGGCCGTGTCCTCAGATTTCAGGCTGCTCAGCTCCATGTAGG12110.10942016348872322No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12090.10923945306182195No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12060.10896838742147004No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG12030.10869732178111811No Hit
GTCTCCAGGCACCCTGTCTTTGTCCCCCGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTGGCAG11760.10625773101795087No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11680.10553488931034576No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC11100.10029428693020873No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11090.1002039317167581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11750.048.3322371
TACGTAT606.6859915E-445.46075470
CCTACAT8700.038.40649870
AATGGTA4800.038.3575170
GTATCAA31550.038.1559451
TTGTCGC400.00268126834.27412437
AACTGTG23150.033.87464570
TATCAAC35750.033.766882
TCAACGC36850.032.9434744
ATCAACG37000.032.8099173
CAACGCA37850.032.2527855
AACGCAG38100.032.0411536
ATCTCGT450.00477353430.46588737
TTTGTCG450.00478737330.44786336
ACGCAGA40400.030.3011957
CTCGGTA450.004931877430.26329827
CGCAGAG40600.030.151938
TCGATCC802.989058E-629.88235532
ACGAGAC1501.8189894E-1229.48004522
AGAGTAC41650.029.06390811
Produced by FastQC (version 0.11.5)