FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188769
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15470.8195201542626173No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7410.39254326716780824No Hit
GTATTACTGTCAGCAGTATGGTAGGTCATTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG7210.38194830719026956No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA4590.24315433148451282No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC4530.2399758434912512No Hit
CAGTAATACACTCCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA4470.2367973554979896No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC4260.22567264752157398No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC3990.21136945155189676No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT3870.20501247556537358No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT3840.20342323156874276No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG3560.1885902876001886No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA3280.17375734363163442No Hit
ATGGTAGGTCATTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA3240.1716383516361267No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC3210.17004910763949588No Hit
GTATGGTAGGTCATTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT3110.16475162765072657No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC3080.16316238365409574No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA2940.15574591166981866No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT2890.153097171675434No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT2820.14938893568329548No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT2820.14938893568329548No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT2800.1483294396855416No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG2630.1393237237046337No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT2590.13720473170912598No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC2580.13667498371024905No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG2580.13667498371024905No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC2520.13349649571698743No Hit
GTGTATTACTGTCAGCAGTATGGTAGGTCATTCACTTTCGGCGGAGGGAC2500.13243699971923356No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGGAGTGTA2470.13084775572260277No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC2390.12660977173158727No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCATGTTTCTGCTGGTACCAGGCTAAGTAGTTGCTGCTAACACTCTGA2370.1255502757338334No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG2360.12502052773495648No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA2350.12449077973607954No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG2340.12396103173720262No Hit
GTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACATGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT2310.1223717877405718No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG2280.120782543743941No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT2240.11866355174843327No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG2220.11760405575067939No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT2160.1144255677574178No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA2150.11389581975854085No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC2100.1112470797641562No Hit
GTAATACACTCCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA2070.1096578357675254No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA2060.10912808776864845No Hit
ACCTACCATACTGCTGACAGTAATACACTCCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT2060.10912808776864845No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG1960.10383060777987912No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT1950.10330085978100217No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG1940.10277111178212525No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA1930.1022413637832483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCAAAT200.00591399254.67131460
TAGTCAC200.0075458151.4058138
GAGAGCG200.00816047950.3978547
GCGCTCC200.00816903250.38450613
GTATCAA5750.036.861181
ATTGACA1753.3069227E-936.04861570
TCGTATA751.0654021E-634.07647761
CTCGTAT751.0798922E-634.01770860
CCACTCG858.09905E-833.9522457
TATCAAC6350.033.899052
CAAGATC400.002949950533.598574
CCCGCTA850.00321963232.9856670
GTGGCCG1501.4563757E-632.71077770
GGTATCA3000.032.5217061
AATGCTA450.00349529332.45378561
TAATGCT450.00352498432.39781660
CAACGCA6500.032.0478675
ATCAACG6500.032.0478673
CGTATAT801.7363382E-632.01121562
AACGCAG6550.031.8032266
Produced by FastQC (version 0.11.5)