FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368975
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTCGGTCAGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAACCGAACTGTGG75890.5543563615113497No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58710.42886100914918096No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAACTCTGTACCAGACCCACTGCCA53070.3876623020873281No Hit
CTCCAGGCACCGTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGC44530.32527986267097647No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43850.32031264267061127No Hit
CATCTATGGTGTCTCAAACAGGGCCACTGGCATCCCTGACAGGTTCAGTGGCAGTGGGTCTGGTACAGAGTTCA39080.285469055315108No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA38160.27874869884402564No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36690.26801073796088315No Hit
GTGTATTACTGTCAGCAGTATGGTCGGTCAGGGACGTTCGGCCAAGGGAC34720.25362040943041325No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGTTGCTGCTAACACTCTGA34610.2528168885480013No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC34380.25113679943023065No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33970.2481418579594222No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32950.24069102795887437No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC32090.23440895560547123No Hit
GTCTGGTACAGAGTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT28820.21052247119195017No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28810.2104494238390036No Hit
GTACCAGACCCACTGCCACTGAACCTGTCAGGGATGCCAGTGGCCCTGTT28450.2078197191329279No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27120.19810442119103708No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAACTCTGTACCAGACCCACTGCCA26190.19131101736700815No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25900.18919264413155829No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24590.17962344089556057No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT24140.1763363100129659No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24130.17626326266001935No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24130.17626326266001935No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23140.16903157471831112No Hit
GTCTCAAACAGGGCCACTGGCATCCCTGACAGGTTCAGTGGCAGTGGGTC22990.16793586442411293No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22550.16472178089446482No Hit
GTCTCCAGGCACCGTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG21530.15727095089391696No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21050.1537646779524827No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20820.1520845888347121No Hit
GTCAGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAACCGAACTGTGG20670.1509888785405139No Hit
GTGCAGCCACAGTTCGGTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20530.1499662155992622No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20040.1463868953048814No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19930.14558337442246938No Hit
GTTTGAGACACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCT19690.14383023795175223No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGATTTCCACCTTGGTCC19290.1409083438338903No Hit
GAGTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA18930.13827863912781463No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC18600.13586807648057855No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18400.1344071294216476No Hit
GTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGC17480.12768677295056519No Hit
GTCCCTGACCGACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGG17300.12637192059752736No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT17280.12622582589163425No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG16780.12257345824430686No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16700.12198907942073448No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT16220.1184828064793002No Hit
GAGTGAACTCTGTACCAGACCCACTGCCACTGAACCTGTCAGGGATGCCA16110.11767928559688817No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT16020.11702185942036926No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15730.11490348618491937No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15700.11468434412607972No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15590.11388082324366772No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15370.11227378147884365No Hit
GAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTA14730.10759875089026462No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14680.10723351412553189No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14610.10672218265490604No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14410.10526123559597508No Hit
GGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAA14360.10489599883124236No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGACAAATCGTGTTGACGCAGTC14160.1034350517723114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTGT350.005006743657.03941770
TACGTAT1750.049.4341670
GGTATCA13950.048.1224021
GTATCAA40350.036.5678671
TCGTATG2200.036.05488640
CGTATGC2300.034.55371541
TATCAAC43450.033.7211382
TCAACGC44350.032.8807684
ATCAACG44600.032.7740483
ATGCCGT2450.032.56485744
CAACGCA45350.032.1557245
ATCTCGT2250.032.06128337
AATGGTA10600.032.01740670
GTACTAG751.8385181E-631.8047541
AACGCAG46200.031.4903666
ACGAGAC2750.029.74385622
ACGCAGA49350.029.5450677
GTACGTA1951.8189894E-1229.32492369
CGCAGAG50050.029.2679778
CGAGACG2800.029.21698823
Produced by FastQC (version 0.11.5)