FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368975
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTCGGTCAGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAACCGAACTGTGG74370.5432531638634744No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58010.4237476944429226No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAACTCTGTACCAGACCCACTGCCA49490.36151134973246407No Hit
CTCCAGGCACCGTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGC45820.3347029712010811No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA38400.28050183531474276No Hit
CATCTATGGTGTCTCAAACAGGGCCACTGGCATCCCTGACAGGTTCAGTGGCAGTGGGTCTGGTACAGAGTTCA38110.2783834620792929No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37340.27275881590240875No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36970.2700560638433865No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34970.255446593254077No Hit
GTGTATTACTGTCAGCAGTATGGTCGGTCAGGGACGTTCGGCCAAGGGAC34630.2529629832538943No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC31620.23097573001698352No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31090.22710422031081648No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGTTGCTGCTAACACTCTGA31020.22659288884019066No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC30740.2245475629576873No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29840.21797330119249803No Hit
GTCTGGTACAGAGTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT28960.21154513413320186No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAACTCTGTACCAGACCCACTGCCA26520.19372158001424422No Hit
GTACCAGACCCACTGCCACTGAACCTGTCAGGGATGCCAGTGGCCCTGTT25910.18926569148450484No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT25680.18758560236673424No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25180.18393323471940687No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23410.17100385324786793No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23200.16946985883599044No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23150.1691046220712577No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23070.16852024324768533No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22620.16523311236509067No Hit
GTCTCAAACAGGGCCACTGGCATCCCTGACAGGTTCAGTGGCAGTGGGTC22110.16150769736481677No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21650.15814751912927555No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21620.15792837707043592No Hit
GTCTCCAGGCACCGTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG21540.15734399824686354No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21150.15449515148194817No Hit
GTTTGAGACACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCT20630.15069668912872772No Hit
GTGCAGCCACAGTTCGGTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20570.1502584050110484No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20390.14894355265801057No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20240.14784784236381235No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGATTTCCACCTTGGTCC19170.14003177559853175No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18740.1368907394218302No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18430.13462627148048723No Hit
GAGTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA18350.13404189265691482No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18030.13170437736262533No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC17450.12746763089172558No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT17400.1271023941269928No Hit
GTCAGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAACCGAACTGTGG16790.12264650559725343No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT16500.12052813236180354No Hit
GTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGC16460.12023594295001735No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG16410.1198707061852846No Hit
GGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAA16290.11899413794992605No Hit
GAGTGAACTCTGTACCAGACCCACTGCCACTGAACCTGTCAGGGATGCCA16210.11840975912635367No Hit
GTCCCTGACCGACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGG16150.11797147500867437No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15910.11621833853795722No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15860.11585310177322448No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15540.11351558647893496No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15050.10993626618455415No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14940.10913274530214212No Hit
GTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGTCT14930.10905969794919558No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGACAAATCGTGTTGACGCAGTC14820.10825617706678355No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT14630.10686827736079912No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14620.10679523000785258No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14420.10533428294892164No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13970.10204715206632699No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13780.1006592523603426No Hit
GTTCAGTGGCAGTGGGTCTGGTACAGAGTTCACTCTCACCATCAGCAGAC13720.1002209682426633No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)