FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858591
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42370.49348292726105913No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25880.30142407735464266No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18810.219079864568811No Hit
GAGTATTAGCAGCTCCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC18330.2134893098110742No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17210.20044468204302163No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGG16340.19031180154462368No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16320.19007886176305133No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT15820.18425536722374217No Hit
GTACTAGTGGCGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAAGGCC15790.18390595755138361No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15570.18134361995408757No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTCCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT15030.17505424585163365No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14690.17109426956490342No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14140.16468842557166333No Hit
GTAATAAACTGCGAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14050.1636401965545877No Hit
GTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGACCA13070.15222614725754172No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT13050.15199320747596937No Hit
GTCTCCGGCCACCGTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG12430.144772074247226No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12240.1425591463222885No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12200.14209326675914377No Hit
GCCTAGAGCCTGAAGATTTCGCAGTTTATTACTGTCAGCAGCGTAGCAAC12070.14057915817892339No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11520.1341733141856833No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11520.1341733141856833No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10890.12683571106615374No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGGCCAGTTGCTACG10750.12520513259514718No Hit
CAGTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGAC10710.12473925303200244No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10550.12287573477942351No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10430.1214780960899893No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTCGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG10280.11973104772819655No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGGC10110.11775105958483144No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9980.11623695100461105No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9860.11483931231517684No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9750.11355814351652882No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9400.10948169733901242No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9400.10948169733901242No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9300.10831699843115057No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA9240.10761817908643348No Hit
GTATTAGCAGCTCCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC9160.106686419960144No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9140.10645348017857163No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8920.10389114258127559No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8870.10330879312734469No Hit
GCTAATACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACACGGTGGCCGGAGA8870.10330879312734469No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT8860.1031923232365585No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC8830.10284291356419996No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8770.10214409421948284No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8730.10167821465633811No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC200.00815566350.420713
GGTATCA8050.040.1164131
TTATCGT350.001527101438.4381717
GTACGGA451.1619365E-437.376981
ACGGGTA755.0298695E-835.8735416
GTATCAA18350.034.8308681
TCCCTAT450.003427253332.59848463
ATCAACG20350.030.8883653
GTTTAGG553.776165E-430.5811651
GTCTAAA553.776165E-430.5811651
TCAACGC20650.030.4378494
TATCAAC20700.030.3731772
CAACGCA20900.030.2363455
AACGCAG21200.030.1255826
GTTACAG500.006173711328.90324259
GCTTGTA704.5739154E-528.8336731
ACGCAGA22300.028.6395667
CGCAGAG23050.027.9993538
AGAGTAC22900.027.88918711
GCAGAGT23000.027.7679279
Produced by FastQC (version 0.11.5)