FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984153
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATAGCCGTGTCCTCGACTCGCAGGCTGCTCATTTGCAAGTACA43660.44363020790466523No Hit
ATATATTACTGTGCGAAGGCCCCAGGAGTGGCTACAGTTGAGATGCGGTACCTCATTCACTGGGGCCGTGGCACC24210.2459983356246437No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23750.2413242656375584No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22620.22984231110406614No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT22170.22526985133409136No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT21940.22293281634054868No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19680.19996890727356414No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19220.1952948372864788No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17880.18167906819366503No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC16800.1707051647457255No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16480.16745363779818787No Hit
CTTATCATACTGCTGACAGTAATAAATTGCAAAATCTTCAGACTGCAGACTGCTGATGGTAAGAGTGAACT15940.16196668607421813No Hit
CATCTATGCTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA15810.160645753251781No Hit
GTATGATAAGTGGCCCTTCACTTTCGGCCCTGGGACCGAAGTGGATATCCAACGAACTGTGGCTGCACCAT15420.15668295478446947No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15150.1539394789224846No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14510.14743642502740933No Hit
CTCCAGGCCCTTCCCTGGAGACTGACGGACCCAACTCATGGCATAGCCGT14420.14652193307341438No Hit
GTCTGGGACAGAGTTCACTCTTACCATCAGCAGTCTGCAGTCTGAAGATT14410.14642032285630383No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGGGTGTTAGCAG13920.14144142221788686No Hit
GGCCTGGAGTGCGTGTCGACTATTAATAGTGGTGGTGGATACACGTCCTA13520.13737701353346482No Hit
CAGTAATATATAGCCGTGTCCTCGACTCGCAGGCTGCTCATTTGCAAGTA13390.13605608071102768No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT13050.13260133332926893No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12950.13158523115816342No Hit
GAGTTCACTCTTACCATCAGCAGTCTGCAGTCTGAAGATTTTGCAATTTATTACTGTCAGCAGTATGATAAGTGG12880.13087395963838955No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12740.12945141659884185No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12200.1239644648748721No Hit
CTCCTGTGTAGACTCTGGATTAATTTTTGACGGCTATGCCATGAGTTGGGTCCGTCAGTCTCCAGGGAAGGGC12200.1239644648748721No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11700.11888395401934454No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11690.11878234380223399No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11570.1175630211969074No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT11270.11451471468359087No Hit
GTGTAGACTCTGGATTAATTTTTGACGGCTATGCCATGAGTTGGGTCCGT11110.11288895120982205No Hit
CTCCTGCAGGGCCAGTCAGGGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT11070.11248251034137985No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC10970.11146640817027434No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT10890.11065352643338992No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10790.10963742426228441No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGACTCTGGATTAATTTTTG10750.10923098339384221No Hit
CTATTAATAGTCGACACGCACTCCAGGCCCTTCCCTGGAGACTGACGGAC10580.10750360970296285No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC10570.10740199948585231No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10500.10669072796607845No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10310.10476013384097799No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10230.10394725210409357No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC10100.1026263192816564No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT10070.10232148863032475No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC9980.1014069966763298No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT9950.10110216602499814No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9910.10069572515655593No Hit
CTATTAATAGTGGTGGTGGATACACGTCCTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT9900.10059411493944538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG13400.063.8330970
CGCGTAT252.599668E-455.0587939
ATATCGT408.59598E-751.4555237
TCGCGTG200.00756497751.3893836
GTATCGC200.00787087650.8766926
CTTTACG200.00791213550.8094523
CGATGTA200.00792167950.7939572
TATCGTA451.915876E-645.8066338
CGTATGC4900.045.11135541
GGTATCA7000.044.991221
CCGTCTT4700.044.53475647
TGCCGTC4850.044.4331345
TCGTATG4750.044.27194240
GCCGTCT4800.043.5408446
ACGAGAC5350.043.0447422
GTATGCC5150.042.3078542
ATGCGCA5500.042.01599529
CATGCGC5500.041.97960328
ACATGCG5450.041.72683727
ATGCCGT5200.041.37338344
Produced by FastQC (version 0.11.5)