FastQCFastQC Report
Wed 8 May 2024
EGAF00004692664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984153
Total Bases72.4 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52800.5365019463437087No Hit
GTAATATATAGCCGTGTCCTCGACTCGCAGGCTGCTCATTTGCAAGTACA47820.4859000582226544No Hit
ATATATTACTGTGCGAAGGCCCCAGGAGTGGCTACAGTTGAGATGCGGTA29680.30157912438411505No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27690.28135869117911544No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27030.27465241684981906No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG24360.247522488881302No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT23130.23502443217670424No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC22890.232585786966051No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22460.22821654763029733No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19750.20068017879333802No Hit
CTCCAGGCCCTTCCCTGGAGACTGACGGACCCAACTCATGGCATAGCCGT19420.19732704162868986No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19180.19488839641803662No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18480.18777568122029806No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC18430.18726763013474532No Hit
GGCCTGGAGTGCGTGTCGACTATTAATAGTGGTGGTGGATACACGTCCTA18380.18675957904919255No Hit
GTATGATAAGTGGCCCTTCACTTTCGGCCCTGGGACCGAAGTGGATATCC16750.17019711366017276No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16670.16938423192328836No Hit
CTTATCATACTGCTGACAGTAATAAATTGCAAAATCTTCAGACTGCAGAC16110.16369405976509752No Hit
CATCTATGCTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTG15720.159731261297786No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT15720.159731261297786No Hit
GTCTGGGACAGAGTTCACTCTTACCATCAGCAGTCTGCAGTCTGAAGATT15660.1591215999951227No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15570.15820710804112775No Hit
GAGTTCACTCTTACCATCAGCAGTCTGCAGTCTGAAGATTTTGCAATTTA15380.1562765139160273No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15290.15536202196203233No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14630.14865574763273595No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14580.1481476965471832No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT14340.14570905133653No Hit
CTCCTGTGTAGACTCTGGATTAATTTTTGACGGCTATGCCATGAGTTGGG13990.1421526937376607No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT13760.13981565874411803No Hit
CTCCTGCAGGGCCAGTCAGGGTGTTAGCAGCAACTTAGCCTGGTACCAGC13400.1361576909281382No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13330.13544641940836435No Hit
GTGTAGACTCTGGATTAATTTTTGACGGCTATGCCATGAGTTGGGTCCGT13300.1351415887570327No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC13240.1345319274543694No Hit
CAGTAATATATAGCCGTGTCCTCGACTCGCAGGCTGCTCATTTGCAAGTA13210.13422709680303774No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA13160.133719045717485No Hit
GTGTGAGGTGCAACTTTTGGAGTCTGGGGGAGGCTTCGTACAGCCTGGGG12870.130772349421279No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT12610.12813048377640468No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12450.12650472030263588No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC12340.1253870079144198No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGACTCTGGATTAATTTTTG12330.12528539769730926No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12040.12233870140110328No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC12040.12233870140110328No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG11940.12132259922999777No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC11910.12101776857866611No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11850.12040810727600282No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGGCCA11640.11827429271668123No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC11590.1177662416311285No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC11240.1142098840322592No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG11040.11217767969004819No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT10990.11166962860449543No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACT10930.11105996730183212No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT10890.11065352643338992No Hit
CTATTAATAGTCGACACGCACTCCAGGCCCTTCCCTGGAGACTGACGGAC10810.10984064469650552No Hit
CTATTAATAGTGGTGGTGGATACACGTCCTATGCAGACTCCGTGAAGGGC10760.10933259361095277No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT10650.10821488122273672No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10540.10709716883452065No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCA10500.10669072796607845No Hit
GTGCAGCCACAGTTCGTTGGATATCCACTTCGGTCCCAGGGCCGAAAGTG10490.1065891177489679No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGG10490.1065891177489679No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGGATATCCACTTCGGTCC10450.1061826768805257No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC10450.1061826768805257No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT10390.10557301557786239No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10360.10526818492653074No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT10210.10374403166987246No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10190.10354081123565137No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC10130.10293114993298806No Hit
GCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC9960.10120377624210869No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA9930.10089894559077704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)