FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518061
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCACTTTGGTAGGTCACAGTACACTTTTGGCCAGGGGA30110.5812056881332507No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21830.4213789495831572No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19980.3856688691100083No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16730.3229349439544764No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA16340.3154068729358126No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14700.2837503691650211No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT12750.246110014071702No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12330.2380028606669871No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGAGTGTTAGCAG12220.23587955858479984No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10730.2071184667442637No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10440.2015206703457701No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10150.19592287394727648No Hit
GTGTTAGCAGCGACTACTTAGCCTGGTACCAGCAAAAACCTGGCCTGGCGCCCAGGCTCCTCATCTATGATGCAT10050.19399259932710627No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGA9730.18781572054256157No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9560.18453425368827223No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9420.18183186922003394No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT9370.18086673190994884No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9170.1770061826696084No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8990.17353168835330202No Hit
CTCCTGCGGGGCCAGTCAGAGTGTTAGCAGCGACTACTTAGCCTGGTACC8890.1716014137331318No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8850.17082930388506373No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8770.16928508418892757No Hit
AAAGTGTACTGTGACCTACCAAAGTGCTGACAATAATACACTGCAAAATC8640.16677572718270628No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8590.16581058987262118No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8300.16021279347412756No Hit
GGCCAAAAGTGTACTGTGACCTACCAAAGTGCTGACAATAATACACTGCA7980.1540359146895829No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC7770.14998233798722546No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG7770.14998233798722546No Hit
ATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA7410.14303334935461268No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT7410.14303334935461268No Hit
GTCAGAGTGTTAGCAGCGACTACTTAGCCTGGTACCAGCAAAAACCTGGC7380.14245426696856162No Hit
ACTTAGCCTGGTACCAGCAAAAACCTGGCCTGGCGCCCAGGCTCCTCATC7340.14168215712049353No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTATTGTCAGCACTTTGGTAGGTCA7320.1412961021964595No Hit
GTGTATTATTGTCAGCACTTTGGTAGGTCACAGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA7210.13917280011427224No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA7190.1387867451902382No Hit
TCTATGATGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT7150.13801463534217012No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7030.13569830579796588No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6920.13357500371577866No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG6800.13125867417157439No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6670.12874931716535312No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT6560.12662601508316587No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6520.12585390523509782No Hit
CATCTATGATGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA6500.12546785031106375No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6490.12527482284904673No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6470.1248887679250127No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA6270.12102821868467226No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6210.11987005391257013No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC6170.11909794406450205No Hit
ACCCTCTCCTGCGGGGCCAGTCAGAGTGTTAGCAGCGACTACTTAGCCTGGTACCAGCAAAAACCTGGCCTGGCG6120.11813280675441694No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6060.11697464198231482No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5760.11118381812180418No Hit
GAGTGTTAGCAGCGACTACTTAGCCTGGTACCAGCAAAAACCTGGCCTGG5750.11099079065978718No Hit
TCATAGATGAGGAGCCTGGGCGCCAGGCCAGGTTTTTGCTGGTACCAGGCTAAGTAGTCGCTGCTAACACTCTGA5570.10751629634348078No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT5510.10635813157137866No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC5460.10539299426129355No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA5430.1048139118752425No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5420.10462088441322547No Hit
GAGCCACCCTCTCCTGCGGGGCCAGTCAGAGTGTTAGCAGCGACTACTTAGCCTGGTACCAGCAAAAACCTGGC5400.10423482948919142No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5380.1038487745651574No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5370.10365574710314035No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5210.10056730771086803No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT5190.10018125278683399No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATT150.002363425768.93626440
CGCGTAT208.658105E-568.7593339
TCGCGTA252.6424628E-454.87204438
GCGTTCA200.007532056451.4425438
ATCGCGT352.3869812E-548.8725837
GCATCGC709.094947E-1248.71466435
AGCATCG1450.046.952134
ACGAGAC2700.046.08472422
TCTCCGA2950.045.5771711
CTTATAC3050.045.211091
ATCTCCG3000.044.81755410
CCCACGA2800.044.42168419
GCCCACG2900.044.04483818
CCGAGCC3050.042.9807714
ACACATC3250.042.4124876
CGAGACT2950.042.1792423
CACGAGA2950.042.17109721
TTATACA3200.042.0286252
TACACAT3300.041.7698755
CGAGCCC3100.041.2032315
Produced by FastQC (version 0.11.5)