FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518061
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCACTTTGGTAGGTCACAGTACACTTTTGGCCAGGGGA27310.5271579987684848No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26340.5084343349528337No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21730.41944867496298704No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19530.3769826333192423No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14270.2754501882982892No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14080.2717826665199658No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13630.2630964307291998No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT13570.26193826595709774No Hit
GTGTTAGCAGCGACTACTTAGCCTGGTACCAGCAAAAACCTGGCCTGGCGCCCAGGCTCCTCATCTATGATGCAT11740.22661424040798284No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11640.2246839657878126No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11550.22294671862965942No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11420.22043736162343816No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGAGTGTTAGCAG11110.2144535103009105No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGA9960.1922553521689531No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9560.18453425368827223No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT9190.17739223759364245No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9100.17565499043548927No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9010.17391774327733608No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8910.17198746865716585No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8490.16388031525245095No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8420.16252912301833183No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8380.16175701317026372No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8330.16079187586017862No Hit
CTCCTGCGGGGCCAGTCAGAGTGTTAGCAGCGACTACTTAGCCTGGTACC8230.15886160124000842No Hit
AAAGTGTACTGTGACCTACCAAAGTGCTGACAATAATACACTGCAAAATC8200.15828251885395733No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA7800.1505614203732765No Hit
ATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA7690.14843811829108927No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7330.14148912965847651No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7320.1412961021964595No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC7280.1405239923483914No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7220.1393658275762893No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT7220.1393658275762893No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG7130.13762858041813608No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7060.13627738818401694No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTATTGTCAGCACTTTGGTAGGTCA7050.13608436072199992No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG7020.13550527833594886No Hit
GTCAGAGTGTTAGCAGCGACTACTTAGCCTGGTACCAGCAAAAACCTGGC7010.13531225087393184No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT6990.1349261959498978No Hit
GGCCAAAAGTGTACTGTGACCTACCAAAGTGCTGACAATAATACACTGCA6940.1339610586398127No Hit
GTGTATTATTGTCAGCACTTTGGTAGGTCACAGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA6840.13203078401964247No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6740.13010050939947226No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6570.12681904254518292No Hit
ACTTAGCCTGGTACCAGCAAAAACCTGGCCTGGCGCCCAGGCTCCTCATC6490.12527482284904673No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA6410.12373060315291055No Hit
TCTATGATGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT6330.1221863834567744No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6140.11851886167845099No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6050.1167816145202978No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC5760.11118381812180418No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5730.11060473573575313No Hit
CATCTATGATGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA5660.10925354350163398No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA5630.10867446111558292No Hit
ACCCTCTCCTGCGGGGCCAGTCAGAGTGTTAGCAGCGACTACTTAGCCTGGTACCAGCAAAAACCTGGCCTGGCG5630.10867446111558292No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT5600.10809537872953186No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA5560.10732326888146376No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5530.10674418649541272No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5510.10635813157137866No Hit
GCCTGGTACCAGCAAAAACCTGGCCTGGCGCCCAGGCTCCTCATCTATGATGCATCCAGCAGGGCCACTGGCATC5480.1057790491853276No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5470.10558602172331057No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC5420.10462088441322547No Hit
TCATAGATGAGGAGCCTGGGCGCCAGGCCAGGTTTTTGCTGGTACCAGGCTAAGTAGTCGCTGCTAACACTCTGA5340.1030766647170893No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT5330.1028836372550723No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5270.10172547248297015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT652.0690277E-763.209870
CGGTACC252.418519E-455.86437645
TTAACGG252.717532E-454.5617735
AACGATC200.0062127954.00957
GGGCGTA252.9233788E-453.7611735
TAACCGT305.3786376E-447.51160453
GTACGTA602.15812E-645.01099469
AACCGTG350.00113351740.8371154
TAACGGC350.001418136839.01856636
TGCTAGG350.001527310838.4342319
TGGTACG606.694756E-638.1781367
GGTATCA7250.037.123261
GGTACGT658.40119E-636.9347568
GTATCAA18450.035.9222871
AAGGCGC502.1586777E-433.6364523
CCTAGTC400.002946767533.616962
CTAGTCC400.002952352233.6039773
TAGAGGC701.1952307E-633.6007355
TATCAAC19800.032.4284822
GTGGTGA4700.032.05319670
Produced by FastQC (version 0.11.5)