FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1961376
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACTTGTCTTCTCTGGTAAAAACAATCGGCCCTCAGGAATCCCA87700.4471350725205162No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC36080.18395249049646778No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35960.18334067511787644No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29510.15045559851859103No Hit
GTATTAGTGCGAGTGGTCGGATGACATATTATGCAGATTCCGTGAAGGGC29380.14979279852511707No Hit
GTAATAAACGGCCGTGTCGTCGGCTCTCAGGCCGCTCATCTGCAAATACA28770.1466827370172777No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT28720.1464278139428646No Hit
CTCCTGTACAACCTCTGGTTTCACCTTCAACAGCCATGCCATGAGCTGGGTCCGCCAGACTCCAGGGAAGGGG27920.14234904475225557No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTAACCCTTGGCTGTTCGGCG27690.14117639860995546No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26900.13714861403422904No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTC26680.13602695250681154No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26060.13286590638408952No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25840.13174424485667205No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25280.12888910642324572No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23620.12042566035273196No Hit
GTATAGGGGCGTGTCCTGGCTTCTGGTGGTACCAGCTTGCATAATAGTTT23510.11986482958902321No Hit
CACTAATACTTGAGACCCATTGCAGCCCCTTCCCTGGAGTCTGGCGGACC23440.11950793728484493No Hit
GACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACAGCCAAGGGTTACCA23200.1182843065276622No Hit
GCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACAGCCA22750.11598999885794462No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22660.11553113732400112No Hit
CAGTAATAGTCACCCTCATCTTCCGCCTGAACCCCAGTGATGGTCAGGGA22540.11491932194540974No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21960.11196221428221821No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21490.10956593738273537No Hit
GTCTGGGGGAGGCCCGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTACAACCTCTGGTTTCACCTTCAACAG20680.10543618357724373No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20540.10472239896888716No Hit
GGGTTACCACTGCTGTCCCGGGAGTTACAGTAATAGTCACCCTCATCTTC20160.10278498360334785No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19980.10186726053546082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT950.050.4250737
CGCGTAT900.045.81194339
TATCGCG1200.045.5497736
GGTATCA16900.045.1940541
AGTATCG2800.042.58325634
GTATCGC1350.040.4292835
CCTAACG1200.039.4231222
ACCGATC6200.039.29354527
TACGTAT1055.513686E-639.27960270
ACGAGAC6200.039.2404422
GTATCAA44200.038.3067251
CTAACGC1250.037.846193
GACCGAT6500.037.4646926
GTATCGG1750.037.03610635
TATACAC6900.036.7296143
CGATCAG6700.036.408629
CCGATCA6750.036.114928
CACGAGA6850.036.00835421
AGACCGA6950.035.5110725
CCCACGA7050.034.50404719
Produced by FastQC (version 0.11.5)