FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1961376
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACTTGTCTTCTCTGGTAAAAACAATCGGCCCTCAGGAATCCCA88850.4529983032320167No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63920.32589365832966244No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC35930.1831877212732286No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32680.16661772143637937No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31720.1617231984076485No Hit
GTATAGGGGCGTGTCCTGGCTTCTGGTGGTACCAGCTTGCATAATAGTTT31320.159683813812344No Hit
CTCCTGTACAACCTCTGGTTTCACCTTCAACAGCCATGCCATGAGCTGGGTCCGCCAGACTCCAGGGAAGGGG29290.14933393699117353No Hit
GTATTAGTGCGAGTGGTCGGATGACATATTATGCAGATTCCGTGAAGGGC28140.14347070627967304No Hit
GTAATAAACGGCCGTGTCGTCGGCTCTCAGGCCGCTCATCTGCAAATACA27510.14025867554206842No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT27300.13918799862953354No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTAACCCTTGGCTGTTCGGCG25900.13205015254596772No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25410.12955190641671968No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTC24740.12613593721958463No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24620.12552412184099326No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24000.12236307571827125No Hit
GTGGTAACCCTTGGCTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCA23620.12042566035273196No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23530.11996679881878844No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23370.11915104498066663No Hit
GGGTTACCACTGCTGTCCCGGGAGTTACAGTAATAGTCACCCTCATCTTC23030.1174175680746578No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23030.1174175680746578No Hit
GTCTGGGGGAGGCCCGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTACAACCTCTGGTTTCACCTTCAACAG22950.1170096911555969No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22790.11619393731747507No Hit
GCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACAGCCA22780.11614295270259246No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21760.11094252198456593No Hit
CACTAATACTTGAGACCCATTGCAGCCCCTTCCCTGGAGTCTGGCGGACC20720.10564012203677418No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20330.10365172205635227No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20070.10232612206940435No Hit
GACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACAGCCAAGGGTTACCA19920.10156135284616515No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19660.1002357528592172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT806.685732E-753.3638870
GGTATCA20450.044.2777251
ACGCTGG16600.035.57592470
GTATCAA48250.034.3817521
GTGGTAT6700.033.786551
CCTAACG1201.8189894E-1233.7684562
TGGTATC6450.033.5066832
CTAACGC1255.456968E-1232.408623
TATCAAC53200.030.9120982
TCAACGC55150.029.8727154
ATCAACG55500.029.74443
GCGATTC25450.029.38462453
GAGCGAT25400.029.00731551
AGCGATT25750.028.83362652
CGATTCT26050.028.79569654
CAACGCA57150.028.6508165
CAGAGCG26250.028.46123349
AACGCAG58250.028.3423276
AGAGCGA26850.027.76594450
GGAATCC27750.027.53208242
Produced by FastQC (version 0.11.5)