FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270129
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48880.38484279943218364No Hit
GCCTTGGGCTGACCCAGGACGGTCATCTTGGTCCCTCCGCCGAATACCAAAGTGCTGCTGCTTGTATATGAGCTG31040.24438462549866985No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29950.2358028200285168No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25070.1973815258135197No Hit
CTGCTATTCCTCACCCTCCTCACTCGGGGCACAGGGTCCTGGGCCCAGTC25040.1971453293326898No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT24290.19124041731194233No Hit
TTATAACTCTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAAC24200.19053182786945264No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG24100.1897445062666863No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22200.17478539581412597No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCATCTTGGTCCCTC21760.1713211807619541No Hit
GATTATTACTGCAGCTCATATACAAGCAGCAGCACTTTGGTATTCGGCGGAGGGACCAAGATGACCGTCCTGGGT21480.16911668027420837No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20280.15966882104101238No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT20140.1585665707971395No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19000.1495911045256033No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18900.14880378292283697No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT18410.14494590706928193No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18310.1441585854665156No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17780.13998578097185405No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17100.13463199407304297No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16600.1306953860592113No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16500.12990806445644498No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTAATTATAACTCTGTCTCCTGGTACCAACAACACCCAGGCAAAGC16480.1297506001358917No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16110.12683751020565628No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15900.12518413483984697No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG15880.12502667051929373No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTAATTATAACTCTGTCTCCTGGTACCAACAACA15750.12400315243569747No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15490.12195611626850501No Hit
GAGTTATAATTACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAT15190.11959415146020601No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15190.11959415146020601No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15010.11817697257522661No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14820.11668106152997058No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT14410.11345304295862861No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14400.11337431079835197No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14200.11179966759281931No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13400.10550109477068864No Hit
GTAATACGCAGCCGTGTCTTCGGCTCTCAGGCTGATCATTTGCAGATCCA13210.10400518372543262No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCGGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC13080.10298166564183638No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13060.10282420132128312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG5250.058.21386370
TCGCGTT451.9579584E-645.66383738
CTAACGC900.044.743193
CGTATGC5750.044.42006741
TCGTATG5250.043.94346640
TAACGCC950.042.388294
TCTCGTA4700.042.2633438
ATCTCGT5100.042.2162837
ATCGCGT1400.041.4983237
CCGTCTT5050.040.99596447
GGTATCA16700.040.9922641
GCGTATG1100.040.69391340
GCTAATC7200.040.5067333
CCTAACG1000.040.267292
CTTATAC9600.039.8494031
ACGAGAC9600.039.50140422
GTATGCC6650.039.04121442
CGAGACT9700.038.7588923
ATGCCGT5500.038.62825444
TGCCGTC5400.038.13156545
Produced by FastQC (version 0.11.5)