FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382304
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC44490.32185394819084656No Hit
CCCCTGTACTTGTCATCTATAATAGAAACTACCGGCCCTCAGGGATCCCG43100.31179827302821955No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT42790.3095556404379934No Hit
GTACAGGGGCCTGTCCTGGCTTCTTCTGGTACCAGCTTGCATAATAGTTT41810.3024660277334074No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC40670.2942189272403176No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA38640.27953330092367523No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC34580.2501620482903905No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33430.24184260481051925No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32850.23764671157719286No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG32770.23706796768294094No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC26970.19510903534967705No Hit
GCCTTGGGCTGACCTAGGACGGAGACCTTGGTCCCAGGTCCGAAGACATA26960.19503669236289556No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC26180.1893939393939394No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25850.18700662083015024No Hit
AGCCAGGACAGGCCCCTGTACTTGTCATCTATAATAGAAACTACCGGCCC25850.18700662083015024No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25290.18295541357038683No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGGTGTGTCTGTGGCCTTGGGA25190.18223198370257193No Hit
CTATTATGCAAGCTGGTACCAGAAGAAGCCAGGACAGGCCCCTGTACTTGTCATCTATAATAGAAACTACCGGCC23780.1720316225663819No Hit
GGATGAGGCTGACTATTACTGTAACTCCCGGGACAGTAGTGGTAGCCATTATGTCTTCGGACCTGGGACCAAGGT23570.17051241984397064No Hit
GTAGTGGTAGCCATTATGTCTTCGGACCTGGGACCAAGGTCTCCGTCCTAGGTCAGCCCAAGGCCAACCCCA22540.16306109220547724No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGGT22540.16306109220547724No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22340.16161423246984744No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21760.15741833923652104No Hit
CTATTACTGTAACTCCCGGGACAGTAGTGGTAGCCATTATGTCTTCGGAC21620.1564055374215802No Hit
GACCTAGGACGGAGACCTTGGTCCCAGGTCCGAAGACATAATGGCTACCA21600.15626085144801724No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21190.15329478898997614No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20840.15076278445262403No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGAGACCTTGGTCCCAGGTCCGAAGACATAATGGCTAC20250.14649454823251615No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGAATCACATGCCAAGGAGACAGCCTCAGAAACTATTATGCAAGCTGGTA20200.1461328332986087No Hit
ACCCTGGTGTGTCTGTGGCCTTGGGACAGACAGTCAGAATCACATGCCAAGGAGACAGCCTCAGAAACTATTATG20190.14606049031182722No Hit
GACTATTACTGTAACTCCCGGGACAGTAGTGGTAGCCATTATGTCTTCGG19660.1422263120124083No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18960.137162302937704No Hit
GGACAGTAGTGGTAGCCATTATGTCTTCGGACCTGGGACCAAGGTCTCCGTCCTAGGTCAGCCCAAGGCCAACCC18860.1364388730698891No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA18560.13426858346644444No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18550.13419624047966294No Hit
CTACTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCCTCCGCCTGAGC18200.1316642359423108No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18090.1308684630877144No Hit
ACCTAGGACGGAGACCTTGGTCCCAGGTCCGAAGACATAATGGCTACCAC17970.13000034724633655No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17130.12392353635669144No Hit
GTAATAGTCAGCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG17120.12385119336990995No Hit
GTCCTGGCTTCTTCTGGTACCAGCTTGCATAATAGTTTCTGAGGCTGTCTCCTTGGCATGTGATTCTGACTGT16750.12117450285899484No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG16510.11943827117623909No Hit
GCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGATGTGTCTCC15940.11531472092969419No Hit
GGACAGGCCCCTGTACTTGTCATCTATAATAGAAACTACCGGCCCTCAGG15840.1145912910618793No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15740.11386786119406439No Hit
CAGTAATAGTCAGCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGA15090.10916556705326758No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGGTGTGTCTGTGG14400.10417390096534483No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13930.10077378058661482No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13890.10048440863948886No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC13860.1002673796791444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)