FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1736865
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT60160.3463711917736842No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA53130.30589596773497074No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC52880.30445659276915593No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC51490.2964536679592254No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC51000.2936324930262283No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50060.28822044315476447No Hit
GATTATTACTGTTGCTCATATGCAGGCAACTACACGGGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT47020.27071764357045597No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCCCGTGTAGTTGCCTGCATATGAGCAACAGTAATAAT46520.2678388936388263No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45320.26092989380291504No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG45070.25949051883710017No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG44020.25344514398067786No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC43820.25229364400802595No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC37880.21809409482026523No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT36130.20801847005956134No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCCCGTGTAGTTGCCTGCATATGAGCAA35740.20577304511289018No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCCCGTGTAGTTGC35530.20456397014160568No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATGACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC33900.19517924536449294No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG33210.19120657045884396No Hit
TCTCTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGTTGCTCATAT32690.18821267052994908No Hit
GTAATAATCAGCCTCATCGTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG32090.18475817061199346No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG31840.18331879564617862No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG30480.17548859583214585No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29270.168522020997602No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG28970.1667947710386242No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28760.16558569606733972No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC28760.16558569606733972No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC28640.1648947960837486No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT28600.1646644960892182No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG26940.15510704631620764No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATGACTATGTCTCCTGGTACCAACAGCA26800.15430099633535135No Hit
GTAATACACAGCCGTGTCCTCGGGAGTCACAGAGTTCAGCTGCAGGGAGA26610.15320707136133205No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG26310.15147982140235425No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG26090.15021317143243718No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC24730.14238297161840444No Hit
CCCCTGAGGGCCGCTTAGTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA24470.14088602165395697No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC23960.1379496967236947No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC23270.13397702181804574No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA22450.12925587193017304No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC22110.12729832197666485No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG22010.12672257199033893No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC21710.1249953220313611No Hit
GGTTATGACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCACTAAG20670.11900752217357134No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT20450.11774087220365427No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT20160.11607119724330907No Hit
GTGTAGTTGCCTGCATATGAGCAACAGTAATAATCAGCCTCATCGTCAGC20080.11561059725424831No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC19930.11474697227475941No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19530.11244397232945565No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT19200.11054399737458005No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA19160.11031369738004969No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19050.10968037239509115No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18510.10657132246893108No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT18480.1063985974730333No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG18220.10490164750858587No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC18040.10386529753319917No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17670.10173502258379322No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG17670.10173502258379322No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA17650.10161987258652802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC501.3460522E-954.047423
TACGTAT1255.7134457E-945.9039670
CCTAACG651.3707904E-841.5797272
TGGGTTG10350.040.88667370
GGTATCA15550.038.6963351
TAGACTC350.001495736338.6030774
GTATCAA40200.036.4958881
TAACGCC601.5522863E-533.777694
AATGGTA8550.033.55552770
TGGTATC6450.033.521642
TATCAAC45150.032.3992652
TCAACGC46650.031.0624454
ATCAACG46700.031.0309723
CAACGCA47700.030.3122315
AACGCAG48050.030.3014836
CTAGACT450.00512737230.0246144
ACGCAGA51600.028.283077
CGCAGAG51750.028.1358098
AGAGTAC52400.027.78679711
GCAGAGT53000.027.599719
Produced by FastQC (version 0.11.5)