FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486853
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT26050.5350691071021437No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16360.33603572330867837No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT15740.3233008731588385No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12170.24997278439282494No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC12020.24689177225979916No Hit
GTATATGAGTAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA11290.23189751321240704No Hit
CTCATATACAGTCAATGAGTCGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTTGGTCAGCCCAAGGCCAACCC10840.2226544768133297No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC10350.21258983717877883No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG10350.21258983717877883No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC10280.2111520315167001No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC9390.19287135952741383No Hit
GTCCTGGGCCCAGTCTGGCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCTCCTG9020.18527152959928356No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT8790.1805473109953107No Hit
GATTATTACTGCTACTCATATACAGTCAATGAGTCGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTTGGTCAG8510.17479608834699592No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGT8140.16719625841886565No Hit
GCCTTGGGCTGACCAAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCGA8060.16555305194791856No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT7680.15774782121091993No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT7680.15774782121091993No Hit
TCCTTGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG7520.15446140826902577No Hit
GACCAAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCGACTCATTGACTGTATATGAGTAGCAGTAATAATCAG7470.15343440422468385No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCATTGGAACCAGCAGTGACGTT7200.14788858238523744No Hit
GTCGATCACCATCTCCTGCATTGGAACCAGCAGTGACGTTGGTGATAATAAATATGTCTCCTGGTACCAACAACA7060.14501297106108002No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG7050.14480757025221164No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTACTCATATACAGTCAATGAGTCGGTCTTCGGAA6900.14172655811918589No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC6550.13453752980879238No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG6420.1318673192935034No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCAAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCGACTCATTGA6400.1314565176757666No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6050.12426748936537312No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC5950.12221348127668925No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG5950.12221348127668925No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGGCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC5920.1215972788500841No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG5900.12118647723234734No Hit
CATATACAGTCAATGAGTCGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTTGGTCAGCCCAAGGCCAACCCCA5670.11646225862837448No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5500.11297044487761194No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG5330.10947863112684937No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA5240.1076300238470339No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA5130.10537061494948166No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT259.0738083E-487.72294670
AGGGGGG5650.085.3940170
GCGCGTA253.2123553E-668.5562238
CCGTCGT650.059.51086447
CGCGTAT1100.059.37063239
AGCGCGT309.670415E-656.93181637
TCGCGTA850.056.45806538
ATCGCGT800.055.50852637
TGCCGTG950.055.1956645
GTCCGGT200.00576053455.0499759
GCGTATG1850.050.2930340
CTCGCTA200.0084197550.0125310
CGTGTTC850.049.83977548
ATGCCGT7050.049.430444
GTCGTAT2450.049.10353539
CGTATGC7850.048.3958241
CTTATAC16750.048.181171
TATACAC17200.047.8851283
GGTATCA6350.047.7908171
TCGTATG7300.047.6775840
Produced by FastQC (version 0.11.5)